Size determination of hyaluronan using a gas-phase electrophoretic mobility molecular analysis
2011 (English)In: Glycobiology, ISSN 0959-6658, E-ISSN 1460-2423, Vol. 22, no 1, 7-11 p.Article in journal (Refereed) Published
Hyaluronan (HA) is a linear non-sulfated polysaccharide mainly found in the extracellular matrix. The size of HA can vary from a fewq saccharides up to at least 25,000 units, reaching molecular weights of 10x103 kDa. HA has mainly biological functions, and both its size and tissue concentration play an important role in many physiological and phatological processes. It is relatively easy ti determine the HA concentration using enzyme-linked binding protein assays, but the molecular weight of HA has so far been shown to be a more challenging task to measure. Here, we present a method for size determination of HA using gas-phase electrophoretic mobility molecular analysis (GEMMA), which utilizes the electrophoretic mobility of molecules in air to estimate the molecular weight of the analyte. We show that this method gives reliable molecular weight estimations of HA in the range 30-2400 kDa, which covers almost its whole biological range. The average measuring time for one GEMMA spectrum is between 5 and 10 min using only 6 pg of HA. In addition the peak area in a GEMMA spectrum can be used to estimate the HA concentration in the sample. The high sensitivity and small sample volumes make GEMMA an excellent tool for both size determination and estimation of concentration of samples with low HA concentration, as is the case for HA extracted from small tissue samples.
Place, publisher, year, edition, pages
Oxford: Oxford University Press, 2011. Vol. 22, no 1, 7-11 p.
Hyaluronan, gas-phase electrophotretic mobility molecular analyzer, HA size, molecular weight
Research subject Medical Biochemistry
IdentifiersURN: urn:nbn:se:umu:diva-46590DOI: 10.1093/glycob/cwr096OAI: oai:DiVA.org:umu-46590DiVA: diva2:439274