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Remarkable heterogeneity in myosin heavy-chain composition of the human young masseter compared with young biceps brachii
Umeå University, Faculty of Medicine, Department of Odontology, Clinical Oral Physiology. Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Odontology, Clinical Oral Physiology.
2012 (English)In: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119X, Vol. 138, no 4, 669-682 p.Article in journal (Refereed) Published
Abstract [en]

Adult human jaw muscles differ from limb and trunk muscles in enzyme-histochemical fibre type composition. Recently, we showed that the human masseter and biceps differ in fibre type pattern already at childhood. The present study explored the myosin heavy-chain (MyHC) expression in the young masseter and biceps muscles by means of gel electrophoresis (GE) and immuno-histochemical (IHC) techniques. Plasticity in MyHC expression during life was evaluated by comparing the results with the previously reported data for adult muscles. In young masseter, GE identified MyHC-I, MyHC-IIa MyHC-IIx and small proportions of MyHC-fetal and MyHC-alpha cardiac. Western blots confirmed the presence of MyHC-I, MyHC-IIa and MyHC-IIx. IHC revealed in the masseter six isomyosins, MyHC-I, MyHC-IIa, MyHC-IIx, MyHC-fetal, MyHC alpha-cardiac and a previously not reported isoform, termed MyHC-IIx'. The majority of the masseter fibres co-expressed two to four isoforms. In the young biceps, both GE and IHC identified MyHC-I, MyHC-IIa and MyHC-IIx. MyHC-I predominated in both muscles. Young masseter showed more slow and less-fast and fetal MyHC than the adult and elderly masseter. These results provide evidence that the young masseter muscle is unique in MyHC composition, expressing MyHC-alpha cardiac and MyHC-fetal isoforms as well as hitherto unrecognized potential spliced isoforms of MyHC-fetal and MyHC-IIx. Differences in masseter MyHC expression between young adult and elderly suggest a shift from childhood to adulthood towards more fast contractile properties. Differences between masseter and biceps are proposed to reflect diverse evolutionary and developmental origins and confirm that the masseter and biceps present separate allotypes of muscle.

Place, publisher, year, edition, pages
2012. Vol. 138, no 4, 669-682 p.
National Category
Dentistry
Identifiers
URN: urn:nbn:se:umu:diva-46738DOI: 10.1007/s00418-012-0985-5OAI: oai:DiVA.org:umu-46738DiVA: diva2:440461
Available from: 2011-09-13 Created: 2011-09-13 Last updated: 2017-12-08Bibliographically approved
In thesis
1. Extra- and intrafusal muscle fibre type compositions of the human masseter at young age.: In perspective of growth and functional maturation of the jaw-face motor system.
Open this publication in new window or tab >>Extra- and intrafusal muscle fibre type compositions of the human masseter at young age.: In perspective of growth and functional maturation of the jaw-face motor system.
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Muscles control body posture and movement by extrafusal and intrafusal (muscle spindle) fibres. The purpose of this thesis was to provide insight into the muscular basis for human jaw function at young age. Extrafusal and intrafusal fibres in the young masseter, and for comparison young biceps, were examined for composition of fibre types and myosin heavy chain (MyHC) isoforms by means of morphological, enzyme-histochemical, biochemical and immuno-histochemical techniques. For evaluation of plasticity during life span the data for young muscles were compared with previous reported data for adult and elderly muscles.

The results showed significant differences in extrafusal fibre types and MyHC expression between young masseter and young biceps and between young masseter and masseter in adults and elderly. Compared with young biceps, young masseter was more intricate in composition of extrafusal MyHC expression. Muscle spindles were larger and more frequent in the masseter than in the biceps. Masseter and biceps muscle spindles showed fundamental similarities but also marked differences in MyHC expression.

The results suggest that the young masseter is specialized in fibre types already at young age and shows a unique fibre type growth pattern. Whereas masseter extrafusal fibres display marked plasticity in fibre types and MyHC isoforms during life span muscle spindles/intrafusal fibres are morphologically mature already at young age and precede extrafusal fibres in growth and maturation. Results showed similarities in intrafusal MyHC expression between young masseter and biceps, but also differences implying muscle specific proprioceptive control. Differences in fibre types and MyHC expression between young masseter and young biceps extrafusal fibres are proposed to reflect diverse evolutionary and developmental origins and accord with the masseter and biceps being separate allotypes of muscle.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2011. 76 p.
Series
Umeå University odontological dissertations, ISSN 0345-7532 ; 120
Keyword
jaw, limb, human, muscle, morphology, fibre type, myosin heavy chain, muscle spindle
National Category
Dentistry
Research subject
Human Anatomy
Identifiers
urn:nbn:se:umu:diva-46667 (URN)978-91-7459-285-6 (ISBN)
Public defence
2011-09-30, Tandläkarhögskolan, byggnad 1D, 9 tr., sal B, Umeå universitet, Umeå, 09:00 (Swedish)
Opponent
Supervisors
Available from: 2011-09-09 Created: 2011-09-09 Last updated: 2012-11-09Bibliographically approved

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