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LC-MS/MS profiling for detection of endogenous steroids and prostaglandins in tissue samples
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Kemiska institutionen.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap.
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2011 (Engelska)Ingår i: Journal of Separation Science, ISSN 1615-9306, E-ISSN 1615-9314, Vol. 34, nr 19, s. 2650-2658Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Roles of steroid hormones, and compounds that can influence their levels in cells, are of increasing interest in e.g. cancer research, partly because resistance to hormone therapies often complicates treatment. To elucidate the processes involved, the hormones and related compounds need to be accurately measured. Reversed-phase liquid chromatography with dynamic multiple reaction monitoring mass spectrometric detection in electrospray mode is capable of providing such measurements. Therefore, LC-MS/MS was developed for sensitive, selective analysis of 11 steroid hormones, cholesterol and two prostaglandins. The effects of the tissue matrix, and solid-phase extraction (SPE) sample clean-up, on the LC-MS/MS signals of the hormones were also investigated. The results show that the developed LC-MS/MS method, following SPE clean-up to reduce matrix interference, can detect selected steroids in extracts of mouse tissues. The method provides linear measurements of the steroids at concentrations up to few ng/μL, and limits of detection in the range 0.03–0.2 pg/μL (for some compounds lower than those of previously reported methods).

Ort, förlag, år, upplaga, sidor
Wiley , 2011. Vol. 34, nr 19, s. 2650-2658
Nyckelord [en]
LC-MS/MS, Prostaglandins, Solid-phase extraction, Steroid hormones, Tissue analysis
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Kemi
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URN: urn:nbn:se:umu:diva-46980DOI: 10.1002/jssc.201100436PubMedID: 21898811OAI: oai:DiVA.org:umu-46980DiVA, id: diva2:441811
Tillgänglig från: 2011-09-19 Skapad: 2011-09-19 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Antti, HenrikWikström, Pernilla

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