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The Riboflavin analog roseoflavin targets an FMN-riboswitch and blocks Listeria monocytogenes growth, but also stimulates virulence gene-expression and infection
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
2011 (English)In: RNA Biology, ISSN 1547-6286, Vol. 8, no 4, 674-680 p.Article in journal (Refereed) Published
Abstract [en]

During recent years, riboswitches have emerged as potential targets for novel antibacterial substances. In this study, we investigated how one flavin analog, roseoflavin, affected the gene-expression, growth and infectivity of the human bacterial pathogen Listeria monocytogenes to determine the potential of this analog to function as an antibacterial substance. The results indicate that roseoflavin has a profound inhibiting effect on the growth of L. monocytogenes at very low concentrations. Also, expression of the gene located downstream of the FMN riboswitch, a riboflavin transporter, was blocked by the addition of roseoflavin. Base-substitution mutations in the FMN riboswitch allowed the bacteria to grow in the presence of roseoflavin, showing that roseoflavin targeted the FMN riboswitch directly. Surprisingly, we found that roseoflavin stimulated L. monocytogenes virulence gene expression and infection abilities in a mechanism independent of the FMN riboswitch. Our results suggest that roseoflavin can block growth but also enhance Listeria virulence.

Place, publisher, year, edition, pages
Georgetown, TX: Landes Bioscience , 2011. Vol. 8, no 4, 674-680 p.
Keyword [en]
riboflavin, roseoflavin, riboswitch, analogs, metabolites, listeria, virulence
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-47407DOI: 10.4161/rna.8.4.15586OAI: diva2:442350
Available from: 2011-09-21 Created: 2011-09-20 Last updated: 2012-04-18Bibliographically approved

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Mansjö, MikaelJohansson, Jörgen
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Department of Molecular Biology (Faculty of Medicine)Molecular Infection Medicine Sweden (MIMS)
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