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Axonal tracing of the normal and regenerating visual pathway of mouse, rat, frog, and fish using manganese-enhanced MRI (MEMRI)
Umeå University, Faculty of Medicine, Department of Pharmacology and Clinical Neuroscience.
Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway.
Molecular Neuroscience, Division of Medical Sciences, Institute of Biomedical Research, University of Birmingham, Birmingham, United Kingdom.
Department of Radiography, Sør-Trondelag University College, Trondheim, Norway.
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2011 (English)In: Journal of Magnetic Resonance Imaging, ISSN 1053-1807, E-ISSN 1522-2586, Vol. 34, no 3, 670-675 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: To assess optic nerve (ON) regeneration after injury by applying manganese-enhanced MRI (MEMRI) in a study of comparative physiology between nonregenerating rat and mouse species and regenerating frog and fish species.

Materials and Methods: The normal visual projections of rats, mice, frogs, and fish was visualized by intravitreal MnCl(2) injection followed by MRI. Rats and mice with ON crush (ONC) were divided into nonregenerating (ONC only), and regenerating animals with peripheral nerve graft (ONC+PNG; rats) or lens injury (ONC+LI; mice) and monitored by MEMRI at 1 and 20 days post-lesion (dpl). Frog and fish with ON transection (ONT) were monitored by MEMRI up to 6 months postlesion (mpl).

Results: Signal intensity profiles of the Mn(2+)-enhanced ON were consistent with ON regeneration in the ONC+PNG and ONC+LI rat and mice groups, respectively, compared with the nonregenerating ONC groups. Furthermore, signal intensity profiles of the Mn(2+)-enhanced ON obtained between 1 mpl and 6 mpl in the fish and frog groups, respectively, were consistent with spontaneous, complete ON regeneration.

Conclusion: Taken together, these results demonstrate that MEMRI is a viable method for serial, in vivo monitoring of normal, induced, and spontaneously regenerating optic nerve axons in different species.

Place, publisher, year, edition, pages
Chicago, IL: Society for Magnetic Resonance Imaging , 2011. Vol. 34, no 3, 670-675 p.
Keyword [en]
CNS injury, regeneration, optic nerve, axon, in vivo imaging, MRI
National Category
Radiology, Nuclear Medicine and Medical Imaging
URN: urn:nbn:se:umu:diva-47386DOI: 10.1002/jmri.22631OAI: diva2:443800
Available from: 2011-09-27 Created: 2011-09-20 Last updated: 2011-09-27Bibliographically approved

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