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Expression, purification, crystallization and preliminary X-ray crystallographic studies of alkyl hydroperoxide reductase (AhpC) from the cyanobacterium Anabaena sp. PCC 7120
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2011 (English)In: Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, ISSN 1744-3091, E-ISSN 1744-3091, Vol. 67, no 10, 1203-1206 p.Article in journal (Refereed) Published
Abstract [en]

Alkyl hydroperoxide reductase (AhpC) is a key component of a large family of thiol-specific antioxidant (TSA) proteins distributed among prokaryotes and eukaryotes. AhpC is involved in the detoxification of reactive oxygen species (ROS) and reactive sulfur species (RSS). Sequence analysis of AhpC from the cyanobacterium Anabaena sp. PCC 7120 shows that this protein belongs to the 1-Cys class of peroxiredoxins (Prxs). It has recently been reported that enhanced expression of this protein in Escherichia coli offers tolerance to multiple stresses such as heat, salt, copper, cadmium, pesticides and UV-B. However, the structural features and the mechanism behind this process remain unclear. To provide insights into its biochemical function, AhpC was expressed, purified and crystallized by the hanging-drop vapour-diffusion method. Diffraction data were collected to a maximum d-spacing of 2.5 Å using synchrotron radiation. The crystal belonged to space group P212121, with unit-cell parameters a = 80, b = 102, c = 109.6 Å. The structure of AhpC from Anabaena sp. PCC 7120 was determined by molecular-replacement methods using the human Prx enzyme hORF6 (PDB entry1prx) as the template.

 

Place, publisher, year, edition, pages
International Union of Crystallography , 2011. Vol. 67, no 10, 1203-1206 p.
Keyword [en]
alkyl hydroperoxide reductase, AhpC, Anabaena sp. PCC 7120
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-47794DOI: 10.1107/S1744309111025747OAI: oai:DiVA.org:umu-47794DiVA: diva2:444575
Available from: 2011-09-29 Created: 2011-09-29 Last updated: 2017-12-08Bibliographically approved

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Mishra, YogeshHall, MichaelJansson, StefanSchröder, Wolfgang PSauer, Uwe H

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Mishra, YogeshHall, MichaelJansson, StefanSchröder, Wolfgang PSauer, Uwe H
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Department of Plant PhysiologyDepartment of ChemistryUmeå Plant Science Centre (UPSC)
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Acta Crystallographica. Section F: Structural Biology and Crystallization Communications
Chemical Sciences

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