umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Preparation of stroma, thylakoid membrane, and lumen fractions from arabidopsis thaliana chloroplasts for proteomic analysis
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2011 (English)In: In Chloroplast Research in Arabidopsis: Methods and Protocols, Volume II. R. / [ed] Paul Jarvis, Springer Science + Business Media, LLC 2011 , 2011, Vol. 775, no 3, 207-222 p.Chapter in book (Refereed)
Abstract [en]

For many studies regarding important chloroplast processes such as oxygenic photosynthesis, fractionation of the total chloroplast proteome is a necessary first step. Here, we describe a method for isolating the stromal, the thylakoid membrane, and the thylakoid lumen subchloroplast fractions from Arabidopsis thaliana leaf material. All three fractions can be isolated sequentially from the same plant material in a single day preparation. The isolated fractions are suitable for various proteomic analyses such as simple mapping studies or for more complex experiments such as differential expression analysis using two-dimensional difference gel electrophoresis (2D-DIGE) or mass spectrometry (MS)-based techniques. Besides this, the obtained fractions can also be used for many other purposes such as immunological assays, enzymatic activity assays, and studies of protein complexes by native-polyacrylamide gel electrophoresis (native-PAGE).

Place, publisher, year, edition, pages
Springer Science + Business Media, LLC 2011 , 2011. Vol. 775, no 3, 207-222 p.
Series
Methods in molecular biology, ISSN 1064-3745 ; 775
Keyword [en]
Arabidopsis thaliana, Lumen isolation, Thylakoid isolation, Stroma isolation, Yeda press
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-47798DOI: 10.1007/978-1-61779-237-3_11PubMedID: 21863445ISBN: 9781617792366 (print)OAI: oai:DiVA.org:umu-47798DiVA: diva2:444585
Available from: 2011-09-29 Created: 2011-09-29 Last updated: 2013-06-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Authority records BETA

Hall, MichaelMishra, YogeshSchröder, Wolfgang P

Search in DiVA

By author/editor
Hall, MichaelMishra, YogeshSchröder, Wolfgang P
By organisation
Department of ChemistryDepartment of Plant Physiology
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
isbn
urn-nbn

Altmetric score

doi
pubmed
isbn
urn-nbn
Total: 246 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf