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An alternative cytokinin biosynthesis pathway
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2000 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 97, no 26, 14778-14783 p.Article in journal (Refereed) Published
Abstract [en]

Studies of de novo cytokinin biosynthesis in isopentenyltransferase (ipt)-transformed Arabidopsis thaliana, involving in vivo deuterium labeling and mass spectrometry, showed that the biosynthetic rate of zeatinriboside-5'-monophosphate was around 66-fold higher than that of isopentenyladenosine-5'-monophosphate (iPMP), the proposed primary product of the Agrobacterium ipt. Double tracer analysis, using [(2)H(6)] isopentenyladenosine and deuterium oxide, provided evidence for an alternative, iPMP-independent, biosynthetic pathway for zeatin-type cytokinins, present in both ipt-expressing and wild-type Arabidopsis thaliana. Reduction of the biosynthetic flux in the alternative pathway by use of mevastatin, an inhibitor for 3-hydroxy-3-methylglutaryl CoA reductase, indicated a terpenoid origin for the side-chain precursor of the iPMP independent pathway.

Place, publisher, year, edition, pages
2000. Vol. 97, no 26, 14778-14783 p.
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Natural Sciences
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URN: urn:nbn:se:umu:diva-48254DOI: 10.1073/pnas.260504097PubMedID: 11114204OAI: oai:DiVA.org:umu-48254DiVA: diva2:448457
Available from: 2011-10-17 Created: 2011-10-12 Last updated: 2017-12-08Bibliographically approved

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