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Microtubules support a disc-like septin arrangement at the plasma membrane of mammalian cells
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Martin Gullberg)
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
2011 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 22, no 23, 4588-4601 p.Article in journal (Refereed) Published
Abstract [en]

Septin family proteins oligomerize through GTP-binding domains into core heteromers, which in turn polymerize at the cleavage furrow of dividing fungal and animal cells. Septin assemblies during the interphase of animal cells remain poorly defined and are the topic of this report. Here we developed protocols for visualization of authentic higher-order assemblies using tagged septins to effectively replace the endogenous gene-product within septin core heteromers in human cells. Our analysis revealed that septins assemble into microtubule-supported disc-like structures at the plasma membrane. In the absence of cell substrate-adhesion, this is the predominant higher-order arrangement in interphase cells and each one of the 7 to 8 septin family members expressed by the two analyzed cell types appears equally represented. However, studies of myeloid and lymphoid cell model systems revealed cell type specific alterations of higher-order septin arrangements in response to substrate-adhesion. Live-cell observations suggested that all higher-order septin assemblies are mutually exclusive with plasma membrane regions undergoing remodeling. The combined data point to a mechanism by which densely arranged cortical microtubules, which are typical for non-adhered spherical cells, support plasma membrane-bound disc-like septin assemblies.

Place, publisher, year, edition, pages
Bethesda: American Society for Cell Biology , 2011. Vol. 22, no 23, 4588-4601 p.
Keyword [en]
filament formation, budding yeast, saccharomyces-cerevisiae, organization, tubulin, actin, localization, cytoskeleton, interphase, dynamics
National Category
Cell and Molecular Biology
Research subject
cellforskning
Identifiers
URN: urn:nbn:se:umu:diva-48430DOI: 10.1091/mbc.E11-09-0754ISI: 000298140000011OAI: oai:DiVA.org:umu-48430DiVA: diva2:450314
Available from: 2011-10-20 Created: 2011-10-20 Last updated: 2017-12-08Bibliographically approved
In thesis
1. Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
Open this publication in new window or tab >>Cytoskeletal filament systems: assembly, regulation, and interplay in mammalian cells
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The cell represents the basic unit of structure and function for all life. The interior of a eukaryotic cell is organized by an extensive array of protein filaments – collectively referred to as the cytoskeleton. These filaments serve diverse essential functions, e.g. to provide mechanical resilience, facilitate intracellular transport, and enable cell polarization, locomotion and division. Here I have explored the mechanisms that regulate synthesis and assembly of two cytoskeletal filament systems – microtubules and septins – and how these interact in human cells. The present thesis is based on three principal discoveries. Firstly, we have found that the microtubule-destabilizing protein Op18/Stathmin also regulates synthesis of tubulin heterodimers, which are the building blocks for microtubules. Secondly, we have unraveled the general rules that govern assembly of mammalian septins into native polymerization-competent heterooligomers. Finally, our combined results point to a non-reciprocal interplay whereby interphase microtubules support a disc-like arrangement of septin filaments, which delineate static plasma membrane regions. I here discuss the physiological significance and implications of these findings.

Place, publisher, year, edition, pages
Umeå: Umeå universitet, Institutionen för molekylärbiologi, 2011. 68 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1450
National Category
Cell and Molecular Biology
Research subject
cellforskning
Identifiers
urn:nbn:se:umu:diva-48508 (URN)978-91-7459-301-3 (ISBN)
Public defence
2011-11-11, Major Groove, Institutionen för Molekylärbiologi, Försörjningsvägen, Byggnad 6L, Umeå Universitet, Umeå, 09:00 (English)
Opponent
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Available from: 2011-10-21 Created: 2011-10-20 Last updated: 2011-10-21Bibliographically approved

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Sellin, Mikael EHolmfeldt, PerStenmark, SonjaGullberg, Martin
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