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Arabidopsis Plants Grown in the Field and Climate Chambers Significantly Differ in Leaf Morphology and Photosystem Components
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

Background:

Plants exhibit phenotypic plasticity and respond to differences in environmental conditions by acclimation. We have systematically compared leaves of Arabidopsis thaliana plants grown in the field and under controlled low, normal and high light conditions in the laboratory to determine their most prominent phenotypic differences.

Results:

Compared to plants grown under field conditions, the ―indoor plants‖ had larger leaves, modified leaf shapes and longer petioles. Their pigment composition also significantly differed; indoor plants had reduced levels of xanthophyll pigments. In addition, Lhcb1 and Lhcb2 levels were up to three times higher in the indoor plants, but differences in the PSI antenna were much smaller, with only the low-abundance Lhca5 protein showing altered levels. Both isoforms of early-light-induced protein (ELIP) were absent in the indoor plants, and they had less non-photochemical quenching (NPQ). The field-grown plants had a high capacity to perform state transitions. Plants lacking ELIPs did not have reduced growth or seed set rates, but their mortality rates were sometimes higher. NPQ levels between natural ecotypes grown under different conditions were not correlated.

Conclusion:

Our results indicate that comparative analysis of field-grown plants with those grown under artificial conditions is important for a full understanding of plant plasticity and adaptation.

Keyword [en]
Arabidopsis thaliana, Carotenoids, Chlorophyll fluorescence, Early light inducible proteins (ELIPs), Field Plants, Indoor Plants, Light harvesting proteins (LHCs)
National Category
Biological Sciences
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-48561OAI: oai:DiVA.org:umu-48561DiVA: diva2:450989
Funder
Swedish Research Council
Available from: 2011-10-24 Created: 2011-10-24 Last updated: 2015-04-29
In thesis
1. Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
Open this publication in new window or tab >>Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Stressresponser i Arabidopsis med olika kapacitet för ”icke-fotokemisk" quenching
Abstract [en]

When light energy input exceeds the capacity for photosynthesis the plant need to dissipate the excess energy and this is done through non-photo-chemical quenching (NPQ). Photochemical quenching (photosynthesis), NPQ and fluorescence are three alternative faiths of excited chlorophylls. PsbS associates to photosystem II and is involved in NPQ.

The results presented in this thesis were generated on Arabidopsis plants and mainly based on wildtype Col-0 together with a mutant deficient in PsbS (npq4) and a transgene overexpressing PsbS (oePsbS). We connect light and herbivore stress and show that the level of PsbS influences the food preference of both a specialist (Plutella) and a generalist (Spodoptera) herbivore as well as oviposition of Plutella. Level of PsbS also affects both metabolomics and transcriptomics of the plant; up-regulation of genes in the jasmonic acid (JA) -pathway and amount of JA has been found in the npq4 plants after herbivory.

Since many experiments were performed in field we have also characterized the field plant and how it differs from the commonly used lab plant. We have also studied the natural variation of NPQ in Arabidopsis plants both in the field and the lab. The results show surprisingly no correlation.

Abstract [sv]

Överskottsenergi kan vara skadligt för en växts membran och fotosynteskomplex. Vid överskott av solenergi blir fotosystemen mättade och växten behöver därför ett sätt för att göra sig av med all överskottsenergi, detta kallas för ”icke-fotokemisk quenching” (NPQ). Fotokemisk quenching (fotosyntes), NPQ och fluoresens är tre alternativa vägar för exalterade klorofyller. PsbS är involverad i NPQ och associerar med fotosystem II.

De resultat som presenteras i denna avhandling kommer från studier av modellväxten Arabidopsis thaliana (Backtrav), i huvudsak gjorda på vildtypen i jämförelse med en mutant som saknar PsbS (npq4) och en transgen som överuttrycker PsbS (oePsbS). Vi har försökt att undersöka kopplingen mellan ljus- och herbivoristress och visar här att mängden PsbS påverkar både en specialist (Plutella) och en generalist (Spodoptera) insekt vid val av föda, samt Plutella även vid äggläggning. Växternas nivå av PsbS visade sig även påverka metabolomet och transkriptomet, och vi fann en uppreglering av gener i biosyntesen för jasmonat samt mer av själva hormonet jasmonat i npq4 växter efter herbivori.

Eftersom vi har gjort många av experimenten ute i fält har vi även karakteriserat en typisk Arabidopsis växt i fält samt hur denna skiljer sig från den vanligt använda lab-växten. Dessutom har vi även undersökt naturlig variation av NPQ av Arabidopsis både i fält och på lab och resultaten visar, till vår förvåning, att det inte går att finna någon korrelation mellan dessa.

Place, publisher, year, edition, pages
Umeå: Umeå Universitet, Institutionen för fysiologisk botanik, 2011. 54 p.
Keyword
Arabidopsis, NPQ, PsbS, photosynthesis, field experiment, metabolomics
National Category
Biological Sciences
Research subject
Physiological Botany; Molecular Biology
Identifiers
urn:nbn:se:umu:diva-48566 (URN)978-91-7459-314-3 (ISBN)
Public defence
2011-11-18, KBC-huset, KB3B1, Umeå Universitet, Umeå, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council
Available from: 2011-10-28 Created: 2011-10-24 Last updated: 2015-04-29Bibliographically approved

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