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Arabidopsis thaliana with reduced capacity for non-photochemical quenching show altered herbivore preference
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

Under natural conditions, plants have to cope with a multitude of stresses, two of those are light-stress and herbivory. Plants have evolved several mechanisms to avoid the damage done by strong and fluctuating light and one important photoprotection mechanism is the qE-type of non-photochemical quenching (NPQ) where the PsbS protein is involved. We have compared

Arabidopsis thaliana wild type and two "photoprotection genotypes", npq4 and oePsbS that, respectively, lack and overexpress PsbS. In dual-choice feeding experiments on field-grown plants with a specialist (Plutella xylostella) and a generalist (Spodoptera littoralis) insect herbivore, both herbivores preferred the plants with higher expression of PsbS. Also both herbivores survived equally well on the different genotypes but for oviposition, female adults of Plutella xylostella preferred plants with lower expression of PsbS. No difference in the amount and composition of the ten most prominent glucosinolates — the most important substances in the Arabidopsis chemical warfare against herbivores — were found between the genotypes. When leaves of the three genotypes, after transfer from a growth chamber to the field, were profiled for changes in composition of metabolites using GC-MS, we found significant differences in metabolite composition. This suggests that differences in herbivore preferences were rather a consequence of changes in the primary metabolism of the plant rather than differences in the composition of typical "defence compounds". In npq4, superoxide accumulated under high light conditions and is likely to directly, or indirectly after dismutation to H2O2, trigger the metabolic change.

National Category
Biological Sciences
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-48559OAI: oai:DiVA.org:umu-48559DiVA: diva2:450993
Available from: 2011-10-24 Created: 2011-10-24 Last updated: 2015-04-29
In thesis
1. Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
Open this publication in new window or tab >>Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Stressresponser i Arabidopsis med olika kapacitet för ”icke-fotokemisk" quenching
Abstract [en]

When light energy input exceeds the capacity for photosynthesis the plant need to dissipate the excess energy and this is done through non-photo-chemical quenching (NPQ). Photochemical quenching (photosynthesis), NPQ and fluorescence are three alternative faiths of excited chlorophylls. PsbS associates to photosystem II and is involved in NPQ.

The results presented in this thesis were generated on Arabidopsis plants and mainly based on wildtype Col-0 together with a mutant deficient in PsbS (npq4) and a transgene overexpressing PsbS (oePsbS). We connect light and herbivore stress and show that the level of PsbS influences the food preference of both a specialist (Plutella) and a generalist (Spodoptera) herbivore as well as oviposition of Plutella. Level of PsbS also affects both metabolomics and transcriptomics of the plant; up-regulation of genes in the jasmonic acid (JA) -pathway and amount of JA has been found in the npq4 plants after herbivory.

Since many experiments were performed in field we have also characterized the field plant and how it differs from the commonly used lab plant. We have also studied the natural variation of NPQ in Arabidopsis plants both in the field and the lab. The results show surprisingly no correlation.

Abstract [sv]

Överskottsenergi kan vara skadligt för en växts membran och fotosynteskomplex. Vid överskott av solenergi blir fotosystemen mättade och växten behöver därför ett sätt för att göra sig av med all överskottsenergi, detta kallas för ”icke-fotokemisk quenching” (NPQ). Fotokemisk quenching (fotosyntes), NPQ och fluoresens är tre alternativa vägar för exalterade klorofyller. PsbS är involverad i NPQ och associerar med fotosystem II.

De resultat som presenteras i denna avhandling kommer från studier av modellväxten Arabidopsis thaliana (Backtrav), i huvudsak gjorda på vildtypen i jämförelse med en mutant som saknar PsbS (npq4) och en transgen som överuttrycker PsbS (oePsbS). Vi har försökt att undersöka kopplingen mellan ljus- och herbivoristress och visar här att mängden PsbS påverkar både en specialist (Plutella) och en generalist (Spodoptera) insekt vid val av föda, samt Plutella även vid äggläggning. Växternas nivå av PsbS visade sig även påverka metabolomet och transkriptomet, och vi fann en uppreglering av gener i biosyntesen för jasmonat samt mer av själva hormonet jasmonat i npq4 växter efter herbivori.

Eftersom vi har gjort många av experimenten ute i fält har vi även karakteriserat en typisk Arabidopsis växt i fält samt hur denna skiljer sig från den vanligt använda lab-växten. Dessutom har vi även undersökt naturlig variation av NPQ av Arabidopsis både i fält och på lab och resultaten visar, till vår förvåning, att det inte går att finna någon korrelation mellan dessa.

Place, publisher, year, edition, pages
Umeå: Umeå Universitet, Institutionen för fysiologisk botanik, 2011. 54 p.
Keyword
Arabidopsis, NPQ, PsbS, photosynthesis, field experiment, metabolomics
National Category
Biological Sciences
Research subject
Physiological Botany; Molecular Biology
Identifiers
urn:nbn:se:umu:diva-48566 (URN)978-91-7459-314-3 (ISBN)
Public defence
2011-11-18, KBC-huset, KB3B1, Umeå Universitet, Umeå, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council
Available from: 2011-10-28 Created: 2011-10-24 Last updated: 2015-04-29Bibliographically approved

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