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Increased levels of COX-2 in oral lichen planus supports an autoimmune cause of the disease
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi.
Umeå universitet, Medicinska fakulteten, Institutionen för medicinsk biovetenskap.
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2012 (Engelska)Ingår i: Journal of the European Academy of Dermatology and Venereology, ISSN 0926-9959, E-ISSN 1468-3083, Vol. 26, nr 11, s. 1415-1419Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Background: Oral lichen planus (OLP) is a chronic inflammatory disease for which the pathogenesis is not fully understood. OLP has autoimmune features and auto immunity has been suggested as a potential cause, whereas WHO has classified OLP as a premalignant condition. Association between chronic inflammation and cancer is known and chronic inflammation is one of the characteristics of OLP. A protein connected to inflammation and suggested to be involved in cancer development is cyclooxygenase-2 (COX-2) which can be inhibited by microRNA-26b (miR-26b).

Objective: The aim was to map levels of COX-2 and miR-26b in OLP lesions to see if there was any correlation between expression of COX-2 and its regulator miR-26b in OLP.

Methods: In biopsies from 20 OLP patients and 20 age and gender-matched controls laser- micro dissection of epithelium was performed. Quantitative RT-PCR, immunohistochemistry and Western blot were used in the analysis.

Results: Levels of COX-2 mRNA were significantly higher while levels of miR-26b were significantly lower in OLP lesions compared to controls. Using immunohistochemistry normal oral mucosa samples did not show any expression of COX-2 while OLP samples expressed the protein. No COX-2 protein was detectable with Western blot.

Conclusion: Increased expression of COX-2 and decreased expression of miR-26b in OLP suggests both to play a role in OLP. COX-2 has been connected to both malignant development and autoimmunity but as malignant development of OLP is quite rare we suggest that the increased levels of COX-2 seen here support an autoimmune cause of the disease.

Ort, förlag, år, upplaga, sidor
Wiley-Blackwell, 2012. Vol. 26, nr 11, s. 1415-1419
Nyckelord [en]
Oral lichen planus, MicroRNA, Cox-2
Nationell ämneskategori
Odontologi Dermatologi och venereologi
Forskningsämne
patologi; odontologi
Identifikatorer
URN: urn:nbn:se:umu:diva-48570DOI: 10.1111/j.1468-3083.2011.04306.xISI: 000310271000013PubMedID: 22017396OAI: oai:DiVA.org:umu-48570DiVA, id: diva2:451247
Tillgänglig från: 2011-10-25 Skapad: 2011-10-25 Senast uppdaterad: 2018-06-08Bibliografiskt granskad
Ingår i avhandling
1. Oral lichen planus: studies of factors involved in differentiation, epithelial mesenchymal transition and inflammation
Öppna denna publikation i ny flik eller fönster >>Oral lichen planus: studies of factors involved in differentiation, epithelial mesenchymal transition and inflammation
2012 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Background: Lichen planus is a chronic inflammation of skin and mucosa with unknown cause. Oral Lichen Planus, OLP, affects around 2% of the population. Autoimmunity has been suggested as a possible cause as the disease has autoimmune features such as female predominance, cyclic nature and cytotoxic T-cell infiltrate. It has been suggested that the intense inflammatory response seen in OLP is caused by factors on the keratinocyte surface triggering the immune system. Chronic inflammation is one of the hallmarks of oral lichen planus and chronic inflammation is connected to increased risk of tumor development. WHO classifies OLP as a potentially malignant condition with increased risk of developing Squamous cell carcinoma of head and neck, SCCHN, but malignant transformation of OLP is a matter of controversy. The aim of these studies was to further elucidate the autoimmune and premalignant character of OLP. Factors involved in malignant transformation, autoimmunity and inflammation were analyzed in normal oral mucosa, OLP and SCCHN. Factors studied were the signal transducers of Transforming growth factor-β the Smad proteins, microRNAs, COX-2, the receptor CXCR-3 and its ligands CXCL-10 and -11 and ELF-3.

Material and methods: In the study on Smad protein expression formalin fixed and paraffin embedded biopsies from normal oral mucosa, OLP and SCCHN was used. For the remaining studies fresh frozen biopsies from OLP and normal controls was used. All of the fresh frozen OLP samples and their controls were micro dissected to be able to analyze the epithelial part only as well as sections of the whole biopsy. Methods used are immunohistochemistry, qRT-PCR and Western blot.

Results: Analyses of smad proteins expression showed a clear increase of smad3 and smad7 in OLP compared to normal oral mucosa. The expressions of smad proteins in the tumors were more heterogeneous. Some of the SCCHN samples showed a similar expression as OLP while others did not. Micro RNA analyzes showed that miR-21 and miR-203 was significantly increased in OLP epithelium compared to normal oral epithelium while the expression of miR-125b and their potential targets p53 and p63 was decreased in OLP. The presence of COX-2 was significantly higher in OLP than normal controls. At the same time the expression of miR-26b, a suggested repressor of COX-2 was decreased in OLP compared to normal mucosa. The receptor CXCR-3 and its ligands CXCL-10 and -11 were increased in OLP. Expressions of the differentiation involved factor ELF-3 mRNA as well as protein were decreased in OLP.

Conclusion: The factors studied are involved in differentiation, malignant transformation and inflammation. Some of the results in these studies indicate a similar expression pattern for OLP and SCCHN. Several of the factors studied are involved in differentiation and their deregulation suggests a disturbed differentiation pattern and this could indicate a premalignant character of OLP but malignant transformation of OLP lesions are relative rare. A lot of these factors are also involved in inflammatory processes and connected to autoimmune diseases and their deregulation in OLP could also support an autoimmune cause of the disease. Based on our studies a suggestion is that the disturbed differentiation pattern triggers the intense immune response directed against the epithelial cells seen in OLP.

Ort, förlag, år, upplaga, sidor
Umeå: Umeå universitet, 2012. s. 60
Serie
Umeå University odontological dissertations, ISSN 0345-7532 ; 122
Nyckelord
oral lichen planus, differentiation, inflammation, autoimmune, premalign
Nationell ämneskategori
Odontologi
Forskningsämne
odontologi; patologi
Identifikatorer
urn:nbn:se:umu:diva-55419 (URN)978-91-7459-441-6 (ISBN)
Disputation
2012-06-08, Betula, by 6M, Norrlands universitetssjukhus, Umeå, 09:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2012-05-16 Skapad: 2012-05-14 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Danielsson, KarinEbrahimi, MaijdWahlin, Ylva-BrittNylander, KarinBoldrup, Linda

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Danielsson, KarinEbrahimi, MaijdWahlin, Ylva-BrittNylander, KarinBoldrup, Linda
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Institutionen för odontologiInstitutionen för medicinsk biovetenskap
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Journal of the European Academy of Dermatology and Venereology
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