Change search
ReferencesLink to record
Permanent link

Direct link
Detection of Puumala and Rift Valley Fever virus by quantitative RT-PCR and virus viability tests in samples of blood dried and stored on filter paper
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases. (Ahlm o Evander)
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
Swedish Defence Research Agency, Department of CBRN Defence and Security, SE-901 82 Umeå, Sweden.
Show others and affiliations
2011 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 178, no 1-2, 186-90 p.Article in journal (Refereed) Published
Abstract [en]

Haemorrhagic fever viruses cause emerging infections worldwide, and blood or serum is the main sample used for diagnosis. However, storage and transportation of such samples from remote areas to regional laboratories may be complicated and expensive. In this study, a novel approach was evaluated for the detection of Puumala hantavirus (PUUV) RNA and Rift Valley fever virus (RVFV) RNA. Whole-blood samples spiked with viable virus particles were tested in parallel with clinical samples from patients with acute haemorrhagic fever with renal syndrome (nephropathia epidemica). Individual blood samples were spotted on filter paper, dried, and used for RNA extraction at later time points. PUUV RNA was detected by RT-PCR after storage at room temperature for up to six weeks. In contrast, only low copy numbers of RVFV RNA were detected after 1-2 days even though viable RVFV was eluted from the dried filter papers after the same time. The use of filter paper to collect and store blood samples for PUUV RNA detection is therefore a simple and reliable procedure. This approach might facilitate sampling and analysis of other RNA viruses from human or animal sources and could be used for field studies in remote areas or in developing countries.

Place, publisher, year, edition, pages
Amsterdam: Elsevier , 2011. Vol. 178, no 1-2, 186-90 p.
National Category
Clinical Medicine
URN: urn:nbn:se:umu:diva-48992DOI: 10.1016/j.jviromet.2011.09.010PubMedID: 21946288OAI: diva2:452541
Available from: 2011-10-31 Created: 2011-10-31 Last updated: 2012-01-02Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Näslund, JonasKerner, AlexanderDrobni, PeterEvander, MagnusAhlm, Clas
By organisation
Infectious DiseasesVirology
In the same journal
Journal of Virological Methods
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 55 hits
ReferencesLink to record
Permanent link

Direct link