Silicon inhibits signaling pathways and cell-cell communication important for osteoclast formation and bone resorption in vitro
(English)Manuscript (preprint) (Other academic)
Silicon containing materials are used in bone regeneration, and some of the materials, e.g. Bioactive glass 45S5 (BG), release silicon (Si) ions to the surrounding tissue after implantation. The role of Si in bone biology is debated; nevertheless findings suggest that Si is beneficial for bone formation. A majority of the experimental studies on Si and bone have focused on osteoblasts. The effects of Si on osteoclast formation and function have not been directly addressed. In the present study, we show that ionic dissolution extract from BG inhibit osteoclast bone resorption in an organ culture system as well as osteoclast formation in a mouse bone marrow system and in the RAW264.7 cell line. Si containing cell culture medium was prepared to address the issue whether or not the inhibitory effects with BG dissolution extract were Si ion dependent. The results suggest that the inhibitory effects of Si act directly on osteoclast precursors, by interactions with the RANK/RANKL/OPG signaling pathway as well as with gap junction intercellular communication. However, regulation via osteoblasts cannot be excluded. The inhibitory effect of Si on osteoclasts could be useful for future therapies or treating bone loss in patients, provided that molecular mechanisms are established.
Bioactive glass, osteoclast, bone resorption, silicon, RANK/RANKL/OPG
Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-49343OAI: oai:DiVA.org:umu-49343DiVA: diva2:455120