Change search
ReferencesLink to record
Permanent link

Direct link
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-2147-7428
1991 (English)In: Nucleic Acids Research, ISSN 0305-1048, E-ISSN 1362-4962, Vol. 19, no 11, 2881-2887 p.Article in journal (Refereed) Published
Abstract [en]

A region of the Pinus contorta chloroplast genome which contains a duplication of the psbA gene was characterized. From previous experiments it was known that the two copies of the psbA gene were located approximately 3.3 kilobase pairs (kbp) apart, that they had the same orientation and that one endpoint of the duplication was 19 base pairs (bp) downstream of the psbA stop codon. In order to determine the size and additional genetic content of the duplicated segment, both copies as well as the intervening DNA were sequenced completely. It was found that the duplicated segment was 1969 bp long, that the two copies were completely identical and were separated by 2431 bp. The duplicated segment carried, in addition to psbA, the 3' exon of the trnK gene, which was partially included in a 124 bp direct repeat. The translocated copy of the duplicated segment was found to be inserted upstream of the trnK(UUU) gene and was immediately followed by a repeated 41 bp stretch from the psbA coding region. The trnK gene was split by a 2509 bp intron which contained an open reading frame of 515 codons. Sequence comparisons of the duplicated segment and its flanking DNA to the corresponding regions of P. sylvestris, a species which lacks the rearrangements found in P. contorta, made it possible to identify 3-9 bp homologies within which recombinations has occurred. A model was derived which would accommodate the conversion of a trnK-psbA locus of the ancestral, P. sylvestris-like organization into the rearranged structure found in P. contorta.

Place, publisher, year, edition, pages
1991. Vol. 19, no 11, 2881-2887 p.
URN: urn:nbn:se:umu:diva-46849DOI: 10.1093/nar/19.11.2881ISI: A1991FT16000009OAI: diva2:456619
Available from: 2011-11-15 Created: 2011-09-16 Last updated: 2015-04-29

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Gustafsson, Petter
By organisation
Department of Plant PhysiologyUmeå Plant Science Centre (UPSC)
In the same journal
Nucleic Acids Research

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 17 hits
ReferencesLink to record
Permanent link

Direct link