Altered expression of miR-21, miR-125b, and miR-203 indicates a role for these microRNAs in oral lichen planus
2012 (English)In: Journal of Oral Pathology & Medicine, ISSN 0904-2512, E-ISSN 1600-0714, Vol. 41, no 1, 90-95 p.Article in journal (Refereed) Published
Background: Oral lichen planus (OLP), which is a chronic inflammatory disease of the oral mucosa with unknown etiology, affects about 2% of the population. MicroRNAs are small non-coding RNAs involved in normal processes such as development and differentiation as well as progression of human diseases. The aim of this study was to investigate the expression of miR-21, miR-125b, and miR-203 and to compare RNA levels of their potential targets, the tumor suppressor p53 and its relative p63, both known to be deregulated in OLP.
Methods: In biopsies from 20 patients with OLP and 20 age- and sex-matched healthy controls, epithelium was laser dissected and analyzed for the expression of miR-21, miR-125b, miR-203, p53, and p63 using qRT/PCR.
Results: Increased expression of miR-21 and miR-203, decreased expression of miR-125, and down-regulation of p53 and ΔNp63 RNA were seen in OLP compared to normal oral mucosa. When comparing microRNA expression to levels of p53 and p63 RNA, a significant negative correlation was seen between ΔNp63 and miR-203 and between miR-21 and p53, respectively.
Conclusion: Results indicate a role for the studied microRNAs in changes seen in OLP.
Place, publisher, year, edition, pages
2012. Vol. 41, no 1, 90-95 p.
miR-125b;miR-203;miR-21;oral lichen planus
Research subject Odontology; Pathology
IdentifiersURN: urn:nbn:se:umu:diva-49757DOI: 10.1111/j.1600-0714.2011.01084.xPubMedID: 21943223OAI: oai:DiVA.org:umu-49757DiVA: diva2:457215