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Reduced levels of E-cadherin correlate with progression of corticotroph pituitary tumours
Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
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2011 (English)In: Clinical Endocrinology, ISSN 0300-0664, E-ISSN 1365-2265, Vol. 75, no 6, 811-818 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: Loss of E-cadherin is an important marker of epithelial tumour progression. The aims of this study were to explore whether E-cadherin expression and localization correlate to corticotroph tumour progression, relate the expression of the E-cadherin gene (CDH1) to immunohistochemical E-cadherin staining pattern, and study whether the E-cadherin levels were correlated to methylation status of the CDH1 promoter region.

Design: Immunohistochemical analyses of E-cadherin protein were performed, as was RT-qPCR of the CDH1 and the POMC genes. Methylation pattern of the promoter region of CDH1 was measured using pyrosequencing of bisulfite-treated DNA.

Patients: Forty-five patients operated at a tertiary referral centre in Oslo, Norway. Adenoma tissue sections and RNA samples from patients with verified Cushing's disease or Nelson's syndrome were collected.

Measurements: Expression of E-cadherin mRNA and protein in pituitary corticotroph adenomas and average percentage of methylated cytosines in a cytosine-phosphate-guanosine island of the CDH1 promoter.

Results: Correlations were observed between tumour progression and both nuclear expression of E-cadherin and reduced CDH1 mRNA. The E-cadherin expression was not determined by the methylation pattern of the CDH1 promoter. Conclusions Corticotroph tumour progression was associated with reduced expression of the epithelial marker E-cadherin.

Place, publisher, year, edition, pages
Oxford: Blackwell Scientific Public. , 2011. Vol. 75, no 6, 811-818 p.
National Category
Endocrinology and Diabetes
URN: urn:nbn:se:umu:diva-50124DOI: 10.1111/j.1365-2265.2011.04109.xISI: 000296912900016OAI: diva2:460395
Available from: 2011-11-30 Created: 2011-11-28 Last updated: 2011-11-30Bibliographically approved

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