IDENTIFICATION OF CASUARINA-FRANKIA STRAINS BY USE OF POLYMERASE CHAIN-REACTION (PCR) WITH ARBITRARY PRIMERS
1992 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 93, no 1, 1-5 p.Article in journal (Refereed) Published
Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers showed polymorphism in the eight different Frankia genomes. Analysis of the AP-PCR products showed 9 polymorphic bands ranging from 4.1-0.60 kb. We conclude that single arbitrary primers can be used to amplify genomic DNA, and that polymorphism can be detected between the amplification products of the different Frankia genomes.
Place, publisher, year, edition, pages
1992. Vol. 93, no 1, 1-5 p.
IdentifiersURN: urn:nbn:se:umu:diva-46821DOI: 10.1016/0378-1097(92)90480-CISI: A1992HV84700001OAI: oai:DiVA.org:umu-46821DiVA: diva2:461270