Persistence of cell-mediated immunity three decades after vaccination with the live vaccine strain of Francisella tularensis
2011 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 41, no 4, 974-980 p.Article in journal (Refereed) Published
The efficacy of many vaccines against intracellular bacteria depends on the generation of cell-mediated immunity, but studies to determine the duration of immunity are usually confounded by re-exposure. The causative agent of tularemia, Francisella tularensis, is rare in most areas and, therefore, tularemia vaccination is an interesting model for studies of the longevity of vaccine-induced cell-mediated immunity. Here, lymphocyte proliferation and cytokine production in response to F. tularensis were assayed in two groups of 16 individuals, vaccinated 1-3 or 27-34 years previously. As compared to naïve individuals, vaccinees of both groups showed higher proliferative responses and, out of 17 cytokines assayed, higher levels of MIP-1β, IFN-γ, IL-10, and IL-5 in response to recall stimulation. The responses were very similar in the two groups of vaccinees. A statistical model was developed to predict the immune status of the individuals and by use of two parameters, proliferative responses and levels of IFN-γ, 91.1% of the individuals were correctly classified. Using flow cytometry analysis, we demonstrated that during recall stimulation, expression of IFN-γ by CD4(+) CCR7(+) , CD4(+) CD62L(+) , CD8(+) CCR7(+) , and CD8(+) CD62L(+) cells significantly increased in samples from vaccinated donors. In conclusion, cell-mediated immunity was found to persist three decades after tularemia vaccination without evidence of decline.
Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 2011. Vol. 41, no 4, 974-980 p.
Cell-mediated immunity, Francisella tularensis, Persistence, Vaccination
Immunology in the medical area
IdentifiersURN: urn:nbn:se:umu:diva-50333DOI: 10.1002/eji.201040923ISI: 000288821000011PubMedID: 21442618OAI: oai:DiVA.org:umu-50333DiVA: diva2:462087