Increased expression of TACI in the NOD mouse results in enhanced plasma cell differentiation and immunoglobulin production
(English)Manuscript (preprint) (Other academic)
B cells have an important pathogenic role in the development of Type 1 diabetes in the NOD mouse. We have previously revealed a novel NOD-specific B cell-related trait, i.e. an increased percentage of TACIhigh-expressing B cells in NOD mice compared with C57BL/6 mice. In the NOD mouse the TACI trait is regulated by genes residing on chromosome 1 and 8, more specifically in the vicinity of the Idd5.4 and Idd22 regions. It has previously been demonstrated that TACI ligation by APRIL influences plasma cell differentiation, immunoglobulin production and isotype switch. In this paper the linkage of the TACI trait to chromosome 1 and 8 was confirmed by analyzing the percentage of TACIhigh-expressing B cells in congenic NOD.B6C1/Idd22 mice. Moreover, the functional concequence of TACI upregulation, with the focus on plasma cell development and immunoglobulin production, was investigated. NOD B cells stimulated with APRIL showed an increased plasma cell differentiation and enhanced IgM, IgG and IgA production compared to B cells from C57BL/6 mice. This supports the hypothesis that increased TACI expression on NOD B cells could contribute to the B cell involvement in the pathogenesis of T1D in the NOD mouse.
NOD, TACI, Idd, APRIL, plasma cell, immunoglobulin
Research subject Immunology
IdentifiersURN: urn:nbn:se:umu:diva-51422OAI: oai:DiVA.org:umu-51422DiVA: diva2:481853