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Alkali-like myosin light chain-1 (myl1) is an early marker for differentiating fast muscle cells in zebrafish
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). (Jonas von Hofsten)
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). (Jonas von Hofsten)
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM). (Jonas von Hofsten)
2011 (English)In: Developmental Dynamics, ISSN 1058-8388, E-ISSN 1097-0177, Vol. 240, no 7, 1856-1863 p.Article in journal (Refereed) Published
Abstract [en]

During myogenesis, muscle precursors become divided into either fast- or slow-twitch fibres, which in the zebrafish occupy distinct domains in the embryo. Genes encoding sarcomeric proteins specific for fast or slow fibres are frequently used as lineage markers. In an attempt to identify and evaluate early definitive markers for cells in the fast-twitch pathway, we analysed genes encoding proteins contributing to the fast sarcomeric structures. The previously uncharacterized zebrafish alkali-like myosin light chain gene (myl1) was found to be expressed exclusively in cells in the fast-twitch pathway initiated at an early stage of fast fibre differentiation. Myl1 was expressed earlier, and in a more fibre type restricted manner, than any of the previously described and frequently used fast myosin light and heavy chain and troponin muscle markers mylz2, mylz3, tnni2, tnnt3a, fMyHC1.3. In summary, this study introduces a novel marker for early differentiating fast muscle cells.

Place, publisher, year, edition, pages
John Wiley & Sons, 2011. Vol. 240, no 7, 1856-1863 p.
Keyword [en]
alkali-like myosin light chain-1;zebrafish;fast muscle cells
National Category
Developmental Biology
URN: urn:nbn:se:umu:diva-51586DOI: 10.1002/dvdy.22677ISI: 000292155800020PubMedID: 21674687OAI: diva2:484447
Available from: 2012-01-27 Created: 2012-01-27 Last updated: 2014-11-14Bibliographically approved
In thesis
1. The role of Six1 in muscle progenitor cells and the establishment of fast-twitch muscle fibres
Open this publication in new window or tab >>The role of Six1 in muscle progenitor cells and the establishment of fast-twitch muscle fibres
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Myogenesis is the process of skeletal muscle tissue formation where committed muscle progenitor cells differentiate into skeletal muscle fibres. Depending on the instructive cues the muscle progenitor cells receive they will differentiate into specific fibre types with different properties. The skeletal muscle fibres can be broadly classified as fast-twitch fibres or slow-twitch fibres, based on their contractile speed. However, subgroups of fast- and slow-twitch fibres with different metabolic properties, endurance and different isoforms of sarcomeric components have also been identified, adding complexity to the process of muscle tissue patterning. The skeletal muscle tissue has the capacity to regenerate throughout life. Upon muscle tissue damage muscle satellite cells are recruited to the area of injury where they proliferate and either form new fibres similar to those damaged, or fuse with existing fibres.

This thesis aims to investigate the process of muscle progenitor cell proliferation and differentiation, as well as the fast-twitch fibre formation and muscle tissue patterning in the zebrafish embryo.

I present results identifying the previously uncharacterised gene myl1, encoding an alkali-like myosin light chain, which is specifically expressed in fast-twitch muscle progenitors before fibre formation. Furthermore, I introduce data showing that the transcription factor six1 is expressed in Pax7+ muscle progenitor cells, which has been reported to contribute to part of the fast-twitch muscle tissue as well as to a pool of quiescent muscle satellite cells. With support from the presented data, I hypothesise that six1 keeps the Pax7+ muscle progenitor cells in a proliferative state and consequently prevents them from differentiating into muscle fibres. In addition, I demonstrate that the zebrafish fast-twitch muscle fibres can be divided into different subgroups that express unique forms of fast myosin heavy chain genes along the anterior-posterior (head-tail) axis, and that this subspecification depends on a balance between RA and Wnt signalling.

Collectively I propose a previously unknown role for Six1 in zebrafish Pax7+ muscle progenitor cell proliferation and differentiation. Furthermore, I present novel data suggesting that distinct regions of the zebrafish body musculature are composed of different fast-twitch fibre types, and that this regionalisation is conserved in adult zebrafish.

Place, publisher, year, edition, pages
Umeå: Umeå Universitet, 2014. 47 p.
Umeå University medical dissertations, ISSN 0346-6612 ; 1684
Myogenesis, zebrafish, muscle fibre, patterning, fmyhc, myl1, Six1, Pax7
National Category
Basic Medicine
urn:nbn:se:umu:diva-95849 (URN)978-91-7601-161-4 (ISBN)
Public defence
2014-12-05, Hörsal B, Unod T9, Norrlands universitetssjukhus, Umeå, 09:00 (English)
Available from: 2014-11-14 Created: 2014-11-06 Last updated: 2014-11-14Bibliographically approved

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Burguière, Anne-CecileNord, Hannavon Hofsten, Jonas
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