Change search
ReferencesLink to record
Permanent link

Direct link
Epidemiology of entamoeba infection in Sudan
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
2011 (English)In: African Journal of Microbiology Research, ISSN 1996-0808, Vol. 5, no 22, 3702-3705 p.Article in journal (Refereed) Published
Abstract [en]

The epidemiology of Entamoeba histolytica infection in Sudan is poorly understood. This is due to the inability to differentiate E. histolytica from the non pathogenic, Entamoeba dispar. Old methods used such as direct microscopy and culturing are insensitive compared to polymerase chain reaction (PCR). In this study, light microscopy and PCR were utilized to study the prevalence of Entamoeba infection in patient attending University of Medical Science and Technology (UMST) hospital, in Khartoum, Sudan. By microscopy 196 stool samples were reported as positive for E. histolytica. PCR detected infections caused by E. histolytica in 54% (106 of 196), and Entamoeba dispar in 51% (100 of 196) of stool samples. By PCR also mixed infections were detected with both E. histolytica and E. dispar in 5% (10 of 196) of stool samples. All 50 negative stool samples examined by microscopy were negative by PCR. The inability to distinguish E. histolytica from the morphologically similar E. dispar in stool samples is the main limitation of microscopic methods used mainly all laboratories in Sudan. All the 196 samples tested were reported positive for E. histolytica by microscopy but in this study it is shown that only 54% (106 of 196) were positive for E. histolytica. The other 51% (100 of 196) were positive for E. dispar, which were misdiagnosed as E. histolytica infections and mistreated with anti-amoebic drugs. Thus, PCR is recommended for detection and accurate identification of Entamoeba species in stool samples.

Place, publisher, year, edition, pages
2011. Vol. 5, no 22, 3702-3705 p.
Keyword [en]
Entamoeba histolytica, Entamoeba dispar, polymerase chain reaction (PCR), Sudan
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-52050ISI: 000298926000016OAI: diva2:495623
Available from: 2012-02-09 Created: 2012-02-08 Last updated: 2012-02-09Bibliographically approved

Open Access in DiVA

No full text

Other links

Fulltext available online

Search in DiVA

By author/editor
Evengård, Birgitta
By organisation
Infectious Diseases
In the same journal
African Journal of Microbiology Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 98 hits
ReferencesLink to record
Permanent link

Direct link