Bacterial cellulose production from cotton-based waste textiles: enzymatic saccharification enhanced by ionic liquid pretreatment
2012 (English)In: Bioresource Technology, ISSN 0960-8524, E-ISSN 1873-2976, Vol. 104, 503-508 p.Article in journal (Refereed) Published
Cotton-based waste textiles were explored as alternative feedstock for production of bacterial cellulose (BC) by Gluconacetobacter xylinus. The cellulosic fabrics were treated with the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). [AMIM]Cl caused 25% inactivation of cellulase activity at a concentration as low as of 0.02 g/mL and decreased BC production during fermentation when present in concentrations higher than 0.0005 g/mL. Therefore, removal of residual IL by washing with hot water was highly beneficial to enzymatic saccharification as well as BC production. IL-treated fabrics exhibited a 5-7-fold higher enzymatic hydrolysis rate and gave a seven times larger yield of fermentable sugars than untreated fabrics. BC from cotton cloth hydrolysate was obtained at an yield of 10.8 g/L which was 83% higher than that from the culture grown on glucose-based medium. The BC from G. xylinus grown on IL-treated fabric hydrolysate had a 79% higher tensile strength than BC from glucose-based culture medium which suggests that waste cotton pretreated with [AMIM]Cl has potential to serve as a high-quality carbon source for BC production.
Place, publisher, year, edition, pages
Oxford: Elsevier, 2012. Vol. 104, 503-508 p.
Bacterial cellulose, Ionic liquid, Cotton-based waste textiles, Enzymatic saccharification, Biorefinery
IdentifiersURN: urn:nbn:se:umu:diva-52425DOI: 10.1016/j.biortech.2011.11.028PubMedID: 22154745OAI: oai:DiVA.org:umu-52425DiVA: diva2:504580