umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Mechanism of estradiol-induced block of voltage-gated K+ currents in rat medial preoptic neurons.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Physiology.
2011 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 6, no 5, e20213- p.Article in journal (Refereed) Published
Abstract [en]

The present study was conducted to characterize possible rapid effects of 17-β-estradiol on voltage-gated K(+) channels in preoptic neurons and, in particular, to identify the mechanisms by which 17-β-estradiol affects the K(+) channels. Whole-cell currents from dissociated rat preoptic neurons were studied by perforated-patch recording. 17-β-Estradiol rapidly (within seconds) and reversibly reduced the K(+) currents, showing an EC(50) value of 9.7 µM. The effect was slightly voltage dependent, but independent of external Ca(2+), and not sensitive to an estrogen-receptor blocker. Although 17-α-estradiol also significantly reduced the K(+) currents, membrane-impermeant forms of estradiol did not reduce the K(+) currents and other estrogens, testosterone and cholesterol were considerably less effective. The reduction induced by estradiol was overlapping with that of the K(V)-2-channel blocker r-stromatoxin-1. The time course of K(+) current in 17-β-estradiol, with a time-dependent inhibition and a slight dependence on external K(+), suggested an open-channel block mechanism. The properties of block were predicted from a computational model where 17-β-estradiol binds to open K(+) channels. It was concluded that 17-β-estradiol rapidly reduces voltage-gated K(+) currents in a way consistent with an open-channel block mechanism. This suggests a new mechanism for steroid action on ion channels.

Place, publisher, year, edition, pages
San Francisco: Public Library of Science , 2011. Vol. 6, no 5, e20213- p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-53900DOI: 10.1371/journal.pone.0020213PubMedID: 21625454OAI: oai:DiVA.org:umu-53900DiVA: diva2:514009
Available from: 2012-04-04 Created: 2012-04-04 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

fulltext(2480 kB)162 downloads
File information
File name FULLTEXT02.pdfFile size 2480 kBChecksum SHA-512
10e6a5b0518d9ebaa06c9004c59b398ed8ebe6e017f3875fdd4610cac37203d4288f236a2c8a12a0a182f9c3d6bfe9abca2191e3b68cf08b7b5369202f5f1f22
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMed

Authority records BETA

Druzin, MichaelMalinina, EvgenyaJohansson, Staffan

Search in DiVA

By author/editor
Druzin, MichaelMalinina, EvgenyaGrimsholm, OlaJohansson, Staffan
By organisation
Physiology
In the same journal
PLoS ONE
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 162 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 98 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf