Change search
ReferencesLink to record
Permanent link

Direct link
Molecular and catalytic characterization of a phi class glutathione transferase from Cathaya argyrophylla
Show others and affiliations
2012 (English)In: Biochemical Systematics and Ecology, ISSN 0305-1978, E-ISSN 1873-2925, Vol. 40, 75-85 p.Article in journal (Refereed) Published
Abstract [en]

Plant phi class glutathione transferases (GSTs) play important roles in stress tolerance and detoxification metabolism. This study reports the cloning, expression and biochemical characteristics of a phi GST gene (CaGSTF) from the endemic and endangered conifer Cathaya argyrophylla. The recombinant CaGSTF showed GSH-conjugating activity towards the substrate NED-Cl and CDNB. Kinetic analysis revealed low catalytic efficiency with a k(cat)/K-m(GSH) value of 9.82 mM(-1)S(-1). The CaGSTF proved to be a thermolabile enzyme, at 40 degrees C the enzyme's activity was nearly abolished. Site-directed mutagenesis revealed that Ser12, Lys42, Ile55, Glu67 and Ser68 of CaGSTF are critical components of glutathione-binding sites that contribute to the enzyme's catalytic activity. Compared to other plant phi GSTs and conifer tau GSTs, CaGSTF showed a narrow substrate spectrum, low catalytic efficiency and thermolability. These atypical properties suggest the enzyme may have a limited functional role in the organism's adaptation to environmental stresses in the subtropical regions. (C) 2011 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
Oxford: Pergamon Press, 2012. Vol. 40, 75-85 p.
Keyword [en]
Cathaya argyrophylla, Gymnosperm, Enzyme characterization, Site-directed mutagenesis, Protein structure
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:umu:diva-54142DOI: 10.1016/j.bse.2011.10.010ISI: 000299712700017OAI: diva2:516722
Available from: 2012-04-19 Created: 2012-04-17 Last updated: 2012-04-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Wang, Xiao-Ru
By organisation
Department of Ecology and Environmental Sciences
In the same journal
Biochemical Systematics and Ecology
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 94 hits
ReferencesLink to record
Permanent link

Direct link