In vivo MRI of olfactory ensheathing cell grafts and regenerating axons in transplant mediated repair of the adult rat optic nerve
2012 (English)In: NMR in Biomedicine, ISSN 0952-3480, E-ISSN 1099-1492, Vol. 25, no 4, 620-631 p.Article in journal (Refereed) Published
The purpose of the present study was to use magnetic resonance imaging (MRI) as a tool for monitoring transplant-mediated repair of the adult rat visual pathway. We labelled rat olfactory ensheathing cells (OECs) using micron-sized particles of iron oxide (MPIO) and transplanted them by: i) intravitreal injection (ivit) and ii) intra-optic nerve (ON) injection (iON) in adult rats with ON crush (ONC) injury. We applied T2-weighted MRI and manganese-enhanced MRI (MEMRI) to visualise transplanted cells and ON axons at specific times after injury and cell engraftment. Our findings demonstrate that ivit MPIO-labelled OECs are unequivocally detected by T2-weighted MRI in vivo and that the T1-weighted 3D FLASH sequence applied for MEMRI facilitates simultaneous visualisation of Mn2+-enhanced regenerating retinal ganglion cell (RGC) axons and MPIO-labelled OEC grafts. Furthermore, analysis of MRI data and ultrastructural findings supports the hypothesis that iON OEC transplants mediate regeneration and remyelination of RGC axons post injury.
Place, publisher, year, edition, pages
Malden, MA, USA: John Wiley & Sons, 2012. Vol. 25, no 4, 620-631 p.
MEMRI, MPIO, manganese, contrast agents, CNS injury, CNS regeneration, optic nerve, visual system, electron probe microanalysis
Biophysics Radiology, Nuclear Medicine and Medical Imaging
IdentifiersURN: urn:nbn:se:umu:diva-54316DOI: 10.1002/nbm.1778ISI: 000302015500016OAI: oai:DiVA.org:umu-54316DiVA: diva2:523457