Change search
ReferencesLink to record
Permanent link

Direct link
Probing the turnover efficiency of photosystem II membrane fragments with different electron acceptors
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2012 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1817, no 8, 1208-1212 p.Article in journal (Refereed) Published
Abstract [en]

In this study we employ isotope ratio membrane-inlet mass spectrometry to probe the turnover efficiency of photosystem II (PSII) membrane fragments isolated from spinach at flash frequencies between 1Hz and 50Hz in the presence of the commonly used exogenous electron acceptors potassium ferricyanide(III) (FeCy), 2,5-dichloro-p-benzoquinone (DCBQ), and 2-phenyl-p-benzoquinone (PPBQ). The data obtained clearly indicate that among the tested acceptors PPBQ is the best at high flash frequencies. If present at high enough concentration, the PSII turnover efficiency is unaffected by flash frequency of up to 30Hz, and at 40Hz and 50Hz only a slight decrease by about 5-7% is observed. In contrast, drastic reductions of the O(2) yields by about 40% and 65% were found at 50Hz for DCBQ and FeCy, respectively. Comparison with literature data reveals that PPBQ accepts electrons from Q(A)(-) in PSII membrane fragments with similar efficiency as plastoquinone in intact cells. Our data also confirm that at high flashing rates O(2) evolution is limited by the reactions on the electron-acceptor side of PSII. The relevance of these data to the evolutionary development of the water-splitting complex in PSII and with regard to the potential of artificial water-splitting catalysts is discussed. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial Photosynthesis.

Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 1817, no 8, 1208-1212 p.
Keyword [en]
Synechocystis-sp PCC-6803, CAB-like proteins, Alga Chlamydomonas-ReinhardTII, Pigment-Binding Protein, Plant Photosystem-I, State Transitions, Arabidopsis-Thaliana, Antenna Proteins, Gene Family, Inducible Protein
National Category
Chemical Sciences
URN: urn:nbn:se:umu:diva-54389DOI: 10.1016/j.bbabio.2012.03.038PubMedID: 22503842Local ID: Volume 1817, Issue 8,OAI: diva2:523651

Available online 6 April 2012

Available from: 2012-04-25 Created: 2012-04-25 Last updated: 2012-12-17Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Shevela, DmitriyMessinger, Johannes
By organisation
Department of Chemistry
In the same journal
Biochimica et Biophysica Acta
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 100 hits
ReferencesLink to record
Permanent link

Direct link