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Disrupted oogenesis in the frog xenopus tropicalis after exposure to environmental progestin concentrations
Umeå University, Faculty of Science and Technology, Department of Chemistry.
2012 (English)In: Biology of Reproduction, ISSN 0006-3363, E-ISSN 1529-7268, Vol. 86, no 4, 126Article in journal (Refereed) Published
Abstract [en]

Levonorgestrel is a synthetic progesterone commonly used in pharmaceuticals, e.g. in contraceptives. It is found in sewage treatment plant effluents at concentrations up to 30 ng/L, and was recently shown to pose a threat to egg laying in fish. Information on the susceptibility of adult frogs to progestin toxicity is lacking. The present study aimed to 1) characterize progestagenic effects on the full cycle of oogenesis (egg development) in frogs, and 2) determine female amphibians' susceptibility to reproductive impacts from progestagenic compounds in the environment. Sexually mature female Xenopus tropicalis were exposed to levonorgestrel via the surrounding water for 7 days (0, 51, or 307 ng/L) or 28 days (0, 1.3, 18, 160, or 1240 ng/L). Their ovaries were analyzed histologically with respect to frequencies of immature (in early meiotic prophase I), previtellogenic, vitellogenic, mature, and atretic oocytes. The 28-day exposure caused reduced proportions of oocytes at immature, vitellogenic and mature stages and increased proportions of previtellogenic oocytes, compared with the control. The lowest tested concentration, 1.3 ng/L, increased the proportions of previtellogenic oocytes and reduced the proportions of vitellogenic oocytes, indicating inhibited vitellogenesis. The present study shows that progestin concentrations found in the aquatic environment impaired oogenesis in adult frogs. Our results indicate that progestagenic effects on oocyte development include interrupted germ cell progression into meiosis and inhibited vitellogenesis. Considering the crucial role of oogenesis in female fertility our results indicate that progestagenic pollutants may pose a threat to reproduction in wild amphibian populations.

Place, publisher, year, edition, pages
Madison: Society for the study of reproduction , 2012. Vol. 86, no 4, 126
Keyword [en]
amphibians, endocrine disruption, levonorgestrel, meiosis, oocyte development, pharmaceuticals, reproductive toxicology, vitellogenesis
National Category
Biological Sciences
URN: urn:nbn:se:umu:diva-54415DOI: 10.1095/biolreprod.111.097378ISI: 000306549500014PubMedID: 22190707OAI: diva2:523736
Available from: 2012-04-26 Created: 2012-04-26 Last updated: 2016-09-05Bibliographically approved

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