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Metabolic profiling reveals metabolic shifts in Arabidopsis plants grown under different light conditions
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).ORCID iD: 0000-0002-7906-6891
2012 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 35, no 10, 1824-1836 p.Article in journal (Refereed) Published
Abstract [en]

Plants have tremendous capacity to adjust their morphology, physiology and metabolism in response to changes in growing conditions. Thus, analysis solely of plants grown under constant conditions may give partial or misleading indications of their responses to the fluctuating natural conditions in which they evolved. To obtain data on growth-condition dependent differences in metabolite levels we compared leaf metabolite profiles of Arabidopsis thaliana growing under three constant laboratory light conditions: 30 (LL), 300 (NL) and 600 (HL) µmol photons m(-2) s(-1) . We also shifted plants to the field and followed their metabolite composition for three days. Numerous compounds showed light-intensity dependent accumulation, including: many sugars and sugar derivatives (fructose, sucrose, glucose, galactose and raffinose); tricarboxylic acid (TCA) cycle intermediates and amino acids (ca. 30% of which were more abundant under HL and 60% under LL). However, the patterns differed after shifting NL plants to field conditions. Levels of most identified metabolites (mainly amino acids, sugars and TCA cycle intermediates) rose after 2 h and peaked after 73 h, indicative of a "biphasic response" and "circadian" effects. The results provide new insight into metabolomic level mechanisms of plant acclimation, and highlight the role of known protectants under natural conditions.

Place, publisher, year, edition, pages
John Wiley & Sons, 2012. Vol. 35, no 10, 1824-1836 p.
Keyword [en]
GC-MS, laboratory and field-grown plants, metabolome analysis, metabolomics
National Category
Environmental Sciences Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-55086DOI: 10.1111/j.1365-3040.2012.02519.xPubMedID: 22497620OAI: oai:DiVA.org:umu-55086DiVA: diva2:525446
Available from: 2012-05-08 Created: 2012-05-08 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
Open this publication in new window or tab >>Stress responses of Arabidopsis plants with a varying level of non-photochemical quenching
2011 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Stressresponser i Arabidopsis med olika kapacitet för ”icke-fotokemisk" quenching
Abstract [en]

When light energy input exceeds the capacity for photosynthesis the plant need to dissipate the excess energy and this is done through non-photo-chemical quenching (NPQ). Photochemical quenching (photosynthesis), NPQ and fluorescence are three alternative faiths of excited chlorophylls. PsbS associates to photosystem II and is involved in NPQ.

The results presented in this thesis were generated on Arabidopsis plants and mainly based on wildtype Col-0 together with a mutant deficient in PsbS (npq4) and a transgene overexpressing PsbS (oePsbS). We connect light and herbivore stress and show that the level of PsbS influences the food preference of both a specialist (Plutella) and a generalist (Spodoptera) herbivore as well as oviposition of Plutella. Level of PsbS also affects both metabolomics and transcriptomics of the plant; up-regulation of genes in the jasmonic acid (JA) -pathway and amount of JA has been found in the npq4 plants after herbivory.

Since many experiments were performed in field we have also characterized the field plant and how it differs from the commonly used lab plant. We have also studied the natural variation of NPQ in Arabidopsis plants both in the field and the lab. The results show surprisingly no correlation.

Abstract [sv]

Överskottsenergi kan vara skadligt för en växts membran och fotosynteskomplex. Vid överskott av solenergi blir fotosystemen mättade och växten behöver därför ett sätt för att göra sig av med all överskottsenergi, detta kallas för ”icke-fotokemisk quenching” (NPQ). Fotokemisk quenching (fotosyntes), NPQ och fluoresens är tre alternativa vägar för exalterade klorofyller. PsbS är involverad i NPQ och associerar med fotosystem II.

De resultat som presenteras i denna avhandling kommer från studier av modellväxten Arabidopsis thaliana (Backtrav), i huvudsak gjorda på vildtypen i jämförelse med en mutant som saknar PsbS (npq4) och en transgen som överuttrycker PsbS (oePsbS). Vi har försökt att undersöka kopplingen mellan ljus- och herbivoristress och visar här att mängden PsbS påverkar både en specialist (Plutella) och en generalist (Spodoptera) insekt vid val av föda, samt Plutella även vid äggläggning. Växternas nivå av PsbS visade sig även påverka metabolomet och transkriptomet, och vi fann en uppreglering av gener i biosyntesen för jasmonat samt mer av själva hormonet jasmonat i npq4 växter efter herbivori.

Eftersom vi har gjort många av experimenten ute i fält har vi även karakteriserat en typisk Arabidopsis växt i fält samt hur denna skiljer sig från den vanligt använda lab-växten. Dessutom har vi även undersökt naturlig variation av NPQ av Arabidopsis både i fält och på lab och resultaten visar, till vår förvåning, att det inte går att finna någon korrelation mellan dessa.

Place, publisher, year, edition, pages
Umeå: Umeå Universitet, Institutionen för fysiologisk botanik, 2011. 54 p.
Keyword
Arabidopsis, NPQ, PsbS, photosynthesis, field experiment, metabolomics
National Category
Biological Sciences
Research subject
Physiological Botany; Molecular Biology
Identifiers
urn:nbn:se:umu:diva-48566 (URN)978-91-7459-314-3 (ISBN)
Public defence
2011-11-18, KBC-huset, KB3B1, Umeå Universitet, Umeå, 10:00 (English)
Opponent
Supervisors
Funder
Swedish Research Council
Available from: 2011-10-28 Created: 2011-10-24 Last updated: 2015-04-29Bibliographically approved

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Johansson Jänkänpää, HannaMishra, YogeshSchröder, Wolfgang PJansson, Stefan
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