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Effects of 17β-estradiol on proliferation, cell viability and intracellular redox status in native human lens epithelial cells
Umeå University, Faculty of Medicine, Department of Clinical Sciences, Ophthalmology.
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2011 (English)In: Molecular Vision, ISSN 1090-0535, E-ISSN 1090-0535, Vol. 17, 1987-1996 p.Article in journal (Refereed) Published
Abstract [en]

PURPOSE: The purpose of this study was to examine the effects of 17β-estradiol on proliferation, cell death and redox status in cultured human lens epithelial cells (HLECs).

METHODS: HLECs were exposed to 17β-estradiol after which cell viability was measured by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) and the number of mitotic and apoptotic cell nuclei was determined after staining with Hoechst 33342. Apoptosis was also determined by measuring caspase-3 activity and propidium iodide was used to determine the proportion of non-viable cells. Pro- and antioxidative effects of 17β-estradiol was investigated by measuring peroxides, superoxides and glutathione, using dichlorofluorescein diacetate (DCFH-DA), dihydroethidium (HET), and monochlorobimane (MCB), respectively. Effects on mitochondrial membrane potential were determined using 5,5',6,6'-tetrachloro-1,1',3,3'- tetraethylbenzimidazolylcarbocyanine iodide (JC-1). The ability of 17β-estradiol to prevent reactive oxygen species (ROS)-production in HLECs after exposure to 25 µM H₂O₂ for 24h was also measured.

RESULTS: This study demonstrates increased mitotic activity in HLECs exposed to physiologic concentrations of 17β-estradiol (1 nM). Pharmacological concentrations of 17β-estradiol caused increased number of apoptotic cell nuclei and caspase-3 activation. Physiologic concentrations of 17β-estradiol (0.1-10 nM) stabilized the mitochondrial membrane potential. Similar or slightly higher concentrations of 17β-estradiol (0.01-1 µM) protected against H₂O₂-induced oxidative stress as evident by decreased levels of peroxides and superoxides.

CONCLUSIONS: The present study demonstrates mitogenic and anti-oxidative effects of 17β-estradiol at physiologic concentrations, whereas pharmacological levels induced oxidative stress and acted pro-apoptotic in cultured lens cells.

Place, publisher, year, edition, pages
2011. Vol. 17, 1987-1996 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-55122PubMedID: 21850173OAI: diva2:525655
ID Pubmedcentral: PMC3154121Available from: 2012-05-08 Created: 2012-05-08 Last updated: 2012-05-25Bibliographically approved

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