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Aggregatibacter actinomycetemcomitans targets NLRP3 and NLRP6 inflammasome expression in human mononuclear leukocytes
Umeå universitet, Medicinska fakulteten, Institutionen för odontologi, Molekylär paradontologi.
2012 (Engelska)Ingår i: Cytokine, ISSN 1043-4666, E-ISSN 1096-0023, Vol. 59, nr 1, s. 124-130Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Periodontitis is an inflammatory condition that destroys the tooth-supporting tissues, as a result of local bacterial infection. Aggregatibacter actinomycetemcomitans is a Gram-negative facultative anaerobic species, highly associated with aggressive periodontitis. Periodontal inflammation is dominated by cytokines of the Interleukin (IL)-1 family. Prior to their secretion by mononuclear cells, IL-1 cytokines are processed by intracellular protein complexes, known as "inflammasomes", which can sense the bacterial challenge. The aim of this study was to investigate which inflammasomes are regulated in mononuclear cells in response to A. actinomycetemcomitans. The D7SS strain and its derivative leukotoxin and cytolethal distending toxin knock-out mutant strains were used to infect human mononuclear cells at a 1:10 cell: bacteria ratio, for 3h. The expression of various inflammasome components in the cells was investigated by TaqMan quantitative real-time polymerase chain reaction (qPCR). The expressions of NOD-like receptor protein (NLRP)1, NLRP2 and Absent In Melanoma (AIM)2 inflammasome sensors, as well as their effector Caspase-1 were not affected. However, NLRP3 was up-regulated, while NLRP6 was down-regulated. This effect was not dependent on the leukotoxin or the cytolethal distending toxin, as demonstrated by the use of specific gene knock-out mutant strains. IL-1β and IL-18 expressions were also up-regulated by the bacterial challenge. In conclusion, A. actinomycetemcomitans enhances NLRP3 and reduces NLRP6 inflammasome expression, irrespective of its major virulence factors, confirming the high pathogenic profile of this species, and providing further insights to the mechanisms of periodontal inflammation.

Ort, förlag, år, upplaga, sidor
Elsevier, 2012. Vol. 59, nr 1, s. 124-130
Nyckelord [en]
Aggregatibacter actinomycetemcomitans, Inflammasome, NLRP3, NLRP6, Mononuclear leukocytes, Interlukin-1β
Nationell ämneskategori
Odontologi
Identifikatorer
URN: urn:nbn:se:umu:diva-56819DOI: 10.1016/j.cyto.2012.03.016PubMedID: 22503597Scopus ID: 000305590700021OAI: oai:DiVA.org:umu-56819DiVA, id: diva2:537699
Tillgänglig från: 2012-06-27 Skapad: 2012-06-27 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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