Change search
ReferencesLink to record
Permanent link

Direct link
Effects of Porphyromonas gingivalis surface-associated material on osteoclast formation
Umeå University, Faculty of Medicine, Department of Odontology, Molecular Periodontology. (Aa-gruppen)
2013 (English)In: Odontology: official journal of The Society of the Nippon Dental University, ISSN 1618-1247, E-ISSN 1618-1255, Vol. 101, no 2, 141-149 p.Article in journal (Refereed) Published
Abstract [en]

Porphyromonas gingivalis strongly correlates with periodontitis, but the underlying mechanisms causing dentoalveolar bone resorption are not fully understood. As contradictory effects of P. gingivalis on osteoclastogenesis have been reported, this study investigates the effect of P. gingivalis extract on osteoclast formation. Osteoclast formation in mouse bone marrow (MBM) cell cultures and RAW 264.7 cells was stimulated by nuclear factor-κB ligand (RANKL) or parathyroid hormone (PTH). Cells were cultured with and without P. gingivalis surface-associated material and phenotypic characteristics were examined using microscopy, flow cytometry, and RT-PCR. P. gingivalis significantly decreased osteoclast formation and the expression of osteoclast phenotypic markers in PTH-stimulated MBM cultures. Additionally, P. gingivalis inhibited expression of osteoclast differentiation factors and stimulated expression of the mouse macrophage marker F4/80. The presence of P. gingivalis in RANKL-stimulated MBM cultures and RAW 264.7 cells inhibited osteoclastogenesis. Interestingly, a transient exposure with P. gingivalis before PTH stimulation increased osteoclastogenesis in MBM cultures. Flow cytometric analyses of cells transiently exposed to P. gingivalis demonstrated an increased proportion of potential osteoclast precursor cells. We conclude that a transient exposure of MBM cultures to P. gingivalis increases the number of osteoclast precursors and osteoclast formation, whereas a prolonged exposure completely abolishes osteoclastogenesis.

Place, publisher, year, edition, pages
2013. Vol. 101, no 2, 141-149 p.
Keyword [en]
Porphyromonas gingivalis, osteoclast formation
National Category
Medical and Health Sciences Dentistry
URN: urn:nbn:se:umu:diva-56821DOI: 10.1007/s10266-012-0068-zISI: 000322740900003PubMedID: 22614338OAI: diva2:537700
Available from: 2012-06-27 Created: 2012-06-27 Last updated: 2013-09-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Johansson, Anders
By organisation
Molecular Periodontology
In the same journal
Odontology: official journal of The Society of the Nippon Dental University
Medical and Health SciencesDentistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 38 hits
ReferencesLink to record
Permanent link

Direct link