umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Influence of pH on the Specific Adhesion of P Piliated Escherichia coli
Umeå University, Faculty of Science and Technology, Department of Physics. Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Science and Technology, Department of Physics.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine).
Umeå University, Faculty of Science and Technology, Department of Physics.
2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 6, e38548- p.Article in journal (Refereed) Published
Abstract [en]

Adhesion to host tissues is an initiating step in a majority of bacterial infections. In the case of Gram-negative bacteria this adhesion is often mediated by a specific interaction between an adhesin, positioned at the distal end of bacterial pili, and its receptor on the surface of the host tissue. Furthermore, the rod of the pilus, and particularly its biomechanical properties, is believed to be crucial for the ability of bacteria to withstand external forces caused by, for example, (in the case of urinary tract infections) urinary rinsing flows by redistributing the force to several pili. In this work, the adhesion properties of P-piliated E. coli and their dependence of pH have been investigated in a broad pH range by both the surface plasmon resonance technique and force measuring optical tweezers. We demonstrate that P piliated bacteria have an adhesion ability throughout the entire physiologically relevant pH range (pH 4.5 - 8). We also show that pH has a higher impact on the binding rate than on the binding stability or the biomechanical properties of pili; the binding rate was found to have a maximum around pH 5 while the binding stability was found to have a broader distribution over pH and be significant over the entire physiologically relevant pH range. Force measurements on a single organelle level show that the biomechanical properties of P pili are not significantly affected by pH.

Place, publisher, year, edition, pages
2012. Vol. 7, no 6, e38548- p.
National Category
Cell and Molecular Biology Other Physics Topics
Identifiers
URN: urn:nbn:se:umu:diva-57365DOI: 10.1371/journal.pone.0038548ISI: 000305343900057OAI: oai:DiVA.org:umu-57365DiVA: diva2:541407
Available from: 2012-07-17 Created: 2012-07-16 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

fulltext(411 kB)190 downloads
File information
File name FULLTEXT02.pdfFile size 411 kBChecksum SHA-512
635523963541eb1a5e6bae102d8e9aeb0eb954409e26d18c2cd3440045d7a25dcd920e3b4141e086a8411de885e353df8594112e33ab7f3c78df10d53f4e9cf6
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Klinth, Jeanna E.Castelain, MickaelUhlin, Bernt EricAxner, Ove
By organisation
Department of PhysicsUmeå Centre for Microbial Research (UCMR)Molecular Infection Medicine Sweden (MIMS)Department of Molecular Biology (Faculty of Medicine)
In the same journal
PLoS ONE
Cell and Molecular BiologyOther Physics Topics

Search outside of DiVA

GoogleGoogle Scholar
Total: 190 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 101 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf