Small Molecule Screening for Inhibitors of the YopH Phosphatase of Yersinia pseudotuberculosis
2012 (English)In: Advances in Yersinia Research, Springer, 2012, Vol. 954, 357-363 p.Chapter in book (Refereed)
Bacterial virulence systems are attractive targets for development of new antibacterial agents. Yersinia spp. utilize the type III secretion (T3S) system to secrete and translocate Yersinia outer proteins (Yop effectors) into the cytosol of the target cell and thereby overcome host defenses to successfully establish an infection. Thus, the Yop effectors constitute attractive targets for drug development. In the present study we apply small molecule screening to identify inhibitors of one of the secreted proteins YopH, a tyrosine phosphatase required for virulence. Characterization of seven inhibitors indicated that both competitive and noncompetitive inhibitors were identified with IC50 values of 6–20 μM.
Place, publisher, year, edition, pages
Springer, 2012. Vol. 954, 357-363 p.
, Advances in experimental medicine and biology, ISSN 0065-2598 ; 954
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:umu:diva-57956DOI: 10.1007/978-1-4614-3561-7_43ISI: 000333327900044PubMedID: 22782782ISBN: 978-1-4614-3560-0ISBN: 978-1-4614-3561-7OAI: oai:DiVA.org:umu-57956DiVA: diva2:546514