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Replication of ribonucleotide-containing DNA templates by yeast replicative polymerases
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
2011 (English)In: DNA Repair, ISSN 1568-7864, E-ISSN 1568-7856, Vol. 10, no 8, 897-902 p.Article in journal (Refereed) Published
Abstract [en]

The major replicative DNA polymerases of S. cerevisiae (Pols α, δ, and ɛ) incorporate substantial numbers of ribonucleotides into DNA during DNA synthesis. When these ribonucleotides are not removed in vivo, they reside in the template strand used for the next round of replication and could potentially reduce replication efficiency and fidelity. To examine if the presence of ribonucleotides in a DNA template impede DNA synthesis, we determined the efficiency with which Pols α, δ, and ɛ copy DNA templates containing a single ribonucleotide. All three polymerases can replicate past ribonucleotides. Relative to all-DNA templates, bypass of ribo-containing templates is slightly reduced, to extents that depend on the identity of the ribo and the sequence context in which it resides. Bypass efficiencies for Pols δ and ɛ were increased by increasing the dNTP concentrations to those induced by cellular stress, and in the case of Pol ɛ, by inactivating the 3'-exonuclease activity. Overall, ribonucleotide bypass efficiencies are comparable to, and usually exceed, those for the common oxidative stress-induced lesion 8-oxo-guanine.

Place, publisher, year, edition, pages
2011. Vol. 10, no 8, 897-902 p.
Keyword [en]
DNA replication, Ribonucleotides, rNMP bypass, Translesion synthesis
National Category
Medical Genetics Pharmacology and Toxicology
URN: urn:nbn:se:umu:diva-58038DOI: 10.1016/j.dnarep.2011.05.009ISI: 000294033800012PubMedID: 21703943OAI: diva2:546627
Available from: 2012-08-24 Created: 2012-08-24 Last updated: 2015-12-09Bibliographically approved

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