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Electron tomography reveals a flared morphology on growing microtubule ends
European Mol Biol Labs, Cell Biol & Biophys Program, D-69117 Heidelberg, Germany .ORCID iD: 0000-0003-3492-3287
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2011 (English)In: Journal of Cell Science, ISSN 0021-9533, E-ISSN 1477-9137, Vol. 124, no Pt 5, 693-698 p.Article in journal (Refereed) Published
Abstract [en]

Microtubules (MTs) exhibit dynamic instability, alternating between phases of growth and shortening, mostly at their uncapped plus ends. Based on results from cryo-electron microscopy it was proposed that growing MTs display mainly curved sheets and blunt ends; during depolymerisation curled 'ramshorns' predominate. Observations of MTs in mitotic cells have suggested that the situation in vivo differs from that in vitro, but so far, a clear comparison between in vivo and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display predominantly a flared morphology. This indicates that MT polymerisation in vivo and in vitro can follow different paths.

Place, publisher, year, edition, pages
2011. Vol. 124, no Pt 5, 693-698 p.
Keyword [en]
Microtubule polymerisation; Plus ends; Electron tomography; Schizosaccharomyces pombe
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:umu:diva-59006DOI: 10.1242/jcs.072967ISI: 000287307000004PubMedID: 21303925OAI: oai:DiVA.org:umu-59006DiVA: diva2:550581
Available from: 2012-09-07 Created: 2012-09-07 Last updated: 2017-12-07Bibliographically approved

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