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Isolation of cDNA sequences coding for a part of human tissue plasminogen activator.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
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1983 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 80, no 2, 349-52 p.Article in journal (Refereed) Published
Abstract [en]

We have isolated a cDNA sequence coding for a part of human tissue plasminogen activator. mRNA coding for tissue plasminogen activator was partially purified, copied into double-stranded cDNA, and cloned into Escherichia coli. Two sets of partially overlapping oligodeoxynucleotide mixtures corresponding to all possible coding sequences for a known portion of the tissue plasminogen activator gene were prepared. One set was used as a probe to screen cDNA containing bacterial clones and both were used as probes in hybridization against purified plasmid DNA. Of 4,200 bacterial clones examined, 1 carried a plasmid that hybridized to both sets of oligonucleotides. This plasmid contained a 370-base-pair cDNA insert, which was shown by nucleotide sequence analysis to code for the cleavage site region in the one-chain form of the human tissue plasminogen activator.

Place, publisher, year, edition, pages
1983. Vol. 80, no 2, 349-52 p.
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Medical and Health Sciences
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URN: urn:nbn:se:umu:diva-59756PubMedID: 6572897OAI: oai:DiVA.org:umu-59756DiVA: diva2:556431
Available from: 2012-09-25 Created: 2012-09-25 Last updated: 2017-12-07

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