Evidence for IFN-beta heterogeneity in a substrain of Namalwa cells.
1985 (English)In: Applied Biochemistry and Biotechnology, ISSN 0273-2289, E-ISSN 1599-0291, Vol. 11, no 2, 111-22 p.Article in journal (Refereed) Published
A substrain of Namalwa cells, denoted substrain B, was grown in fermentors up to the 100-L scale, and was induced with Sendai virus to produce interferon (IFN). The titer of the crude IFN varied extensively between different batches; part of the variation was caused by a differential expression of IFN-alpha and IFN-beta. More than 80% of the IFN activity was IFN-beta by several criteria. A two-step purification procedure was developed and the resulting preparation had a specific activity of approximately 10(6) U/mg protein. The IFN-beta type was found to be heterogeneous, and could be separated into several components, which probably represented post-translational modifications of one molecule.
Place, publisher, year, edition, pages
1985. Vol. 11, no 2, 111-22 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-59765PubMedID: 2992372OAI: oai:DiVA.org:umu-59765DiVA: diva2:556442