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Hormonal regulation of expression of tissue type plasminogen activator and plasminogen activator inhibitor type 1 in cultured rat granulosa cells.
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
1994 (English)In: Science in China. Series B, Chemistry, life sciences & earth sciences, ISSN 1001-652X, Vol. 37, no 3, 271-9 p.Article in journal (Refereed) Published
Abstract [en]

In the present study, gonadotropin and gonadotropin-releasing hormone (GnRH) regulation of tPA and PAI-1 expression in PMSG-primed granulosa cells has been investigated. (i) Addition of gonadotropins (FSH and LH) and GnRH agonist (GnRHa) or PMA to the culture increases tPA activity; FSH (or LH) plus GnRHa (or PMA) in the culture further enhances the enzyme production to such an extent that a more obvious effect than the additive effect caused by these hormones used alone has been observed; (ii) in contrast, FSH and LH decrease PAI-1 activity, whereas GnRHa and PMA alone markedly increase PAI-1 mRNA level and PAI-1 activity. Because FSH and LH stimulate tPA production and have no significant effect on PAI-1 mRNA induction, the observed inhibition of PAI-1 activity by gonadotropins may be due to the occurrence of neutralization of PA and PAI-1 proteins in the conditioned media by the formation of complexes between PA and PAI-1; (iii) increases in PAI-1 mRNA level and activity by GnRH and PMA are completely inhibited by the co-addition of FSH or LH to the culture. It is, therefore, suggested that the mechanism of gonadotropin and GnRHa regulation of tPA and PAI-1 expression in granulosa cells is different.

Place, publisher, year, edition, pages
1994. Vol. 37, no 3, 271-9 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-59911PubMedID: 8068191OAI: diva2:557012
Available from: 2012-09-26 Created: 2012-09-26 Last updated: 2012-09-26

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