Elevated CpxR~P levels repress the Ysc-Yop type III secretion system of Yersinia pseudotuberculosis
2012 (English)In: Research in Microbiology, ISSN 0923-2508, Vol. 163, no 8, 518-530 p.Article in journal (Refereed) Published
One way that Gram-negative bacteria respond to extracytoplasmic stress is through the CpxA-CpxR system. An activated CpxA sensor kinase phosphorylates the CpxR response regulator to instigate positive auto-amplification of Cpx pathway activation, as well as synthesis of various bacterial survival factors. In the absence of CpxA, human enteropathogenic Yersinia pseudotuberculosis accumulates high CpxR~P levels aided by the action of low molecular weight phosphodonors such as acetyl~P. Critically, these bacteria are also defective for plasmid encoded Ysc-Yop-dependent type III synthesis and secretion, an essential determinant of virulence. Herein, we investigated whether elevated CpxR~P levels account for lost Ysc-Yop function. Decisively, reducing CpxR~P in Yersinia defective for CpxA phosphatase activity - through incorporating second-site suppressor mutations in ackA-pta or cpxR - dramatically restored Ysc-Yop T3S function. Moreover, the repressive effect of accumulated CpxR~P is a direct consequence of binding to the promoter regions of the T3S genes. Thus, Cpx pathway activation has two consequences in Yersinia; one, to maintain quality control in the bacterial envelope, and the second, to restrict ysc-yop gene expression to those occasions where it will have maximal effect.
Place, publisher, year, edition, pages
Elsevier, 2012. Vol. 163, no 8, 518-530 p.
Extracytoplasmic stress, CpxA, AckA, Pta, virulence
Microbiology Microbiology in the medical area
Research subject Microbiology; Molecular Biology
IdentifiersURN: urn:nbn:se:umu:diva-60454DOI: 10.1016/j.resmic.2012.07.010OAI: oai:DiVA.org:umu-60454DiVA: diva2:560353
FunderSwedish Research Council