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Interplay between HEAT SHOCK PROTEIN 90 and HY5 Controls PhANG expression in response to the GUN5 Plastid Signal
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Department of Plant Physiology. Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC).
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
Umeå University, Faculty of Science and Technology, Umeå Plant Science Centre (UPSC). Umeå University, Faculty of Science and Technology, Department of Plant Physiology.
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2012 (English)In: Molecular Plant, ISSN 1674-2052, E-ISSN 1752-9867, Vol. 5, no 4, 901-913 p.Article in journal (Refereed) Published
Abstract [en]

The presence of genes encoding organellar proteins in different cellular compartments necessitates a tight coordination of expression by the different genomes of the eukaryotic cell. This coordination of gene expression is achieved by organelle-to-nucleus or retrograde communication. Stress-induced perturbations of the tetrapyrrole pathway trigger large changes in nuclear gene expression in plants. Recently, we identified HSP90 proteins as ligands of the putative plastid signal Mg-ProtoIX. In order to investigate whether the interaction between HSP90 and Mg-ProtoIX is biologically relevant, we produced transgenic lines with reduced levels of cytosolic HSP90 in wild-type and gun5 backgrounds. Our work reveals that HSP90 proteins respond to the tetrapyrrole-mediated plastid signal to control expression of photosynthesis-associated nuclear genes (PhANG) during the response to oxidative stress. We also show that the hy5 mutant is insensitive to tetrapyrrole accumulation and that Mg-ProtoIX, cytosolic HSP90, and HY5 are all part of the same signaling pathway. These findings suggest that a regulatory complex controlling gene expression that includes HSP90 proteins and a transcription factor that is modified by tetrapyrroles in response to changes in the environment is evolutionarily conserved between yeast and plants.

Place, publisher, year, edition, pages
Oxford University Press, 2012. Vol. 5, no 4, 901-913 p.
Keyword [en]
abiotic/environmental stress, cell signaling, organelle biogenesis/function
National Category
Botany
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-60509DOI: 10.1093/mp/ssr112ISI: 000306668400014OAI: oai:DiVA.org:umu-60509DiVA: diva2:561188
Available from: 2012-10-17 Created: 2012-10-15 Last updated: 2017-12-07Bibliographically approved
In thesis
1. Coordination of two different genomes in response to light and stress
Open this publication in new window or tab >>Coordination of two different genomes in response to light and stress
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Hur två olika genom samarbetar under ljus och stress respons
Abstract [en]

About 1.5 billion years ago, a photosynthetic bacteria was engulfed by a eukaryotic cell which initiated a symbiotic relationship and the evolution of the chloroplast as we know it today. The chloroplast has retained its own genome encoding for a number of proteins required for the function of the chloroplast. However, during the evolution most genes were transferred to the nucleus and the chloroplast is thus dependent on the nucleus to provide the majority of proteins necessary for its function. The distribution of genes encoding plastid proteins between two different cellular compartments requires a tight communication in order to coordinate gene expression during different growth conditions. The focus of my PhD studies has been to elucidate signalling pathways between the chloroplast and nucleus that enables the coordination of these two genomes in response to light and stress. The results in this thesis demonstrate that chloroplast retrograde signals triggered by changes in tetrapyrrole levels are important both in response to day-night cycles and during the response to stress. We identified a cytosolic regulatory complex and a novel mechanism that could explain how the tetrapyrrole-mediated signal can be transduced from the plastid to the nucleus and regulate nuclear gene expression in response to changes in the environment. My work further demonstrates that the tetrapyrrole-triggered plastid signalling pathway integrates with the circadian clock in order to fine-tune nuclear gene expression during photoperiodic conditions. These findings provide novel insight into how clock components and plastid signals converge in order to obtain the proper output. I have also examined the regulation of nuclear gene expression in response to redox and early-light signals by identifying transcription factors responding to these signals. My work demonstrates a novel mechanism by which redox-regulation of specific transcription factors directly links cellular redox status to gene regulation. The identified transcription factors were further shown to regulate nuclear genes encoding plastid proteins and they are of particular importance for anterograde control during the early light response and establishment of photomorphogenic growth.

Place, publisher, year, edition, pages
Umeå: Umeå University, 2016. 100 p.
Keyword
Arabidopsis thaliana, chloroplast, cellular communication, tetrapyrrole, circadian clock, oxidative stress, light signalling, gene regulation
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-125373 (URN)978-91-7601-550-6 (ISBN)
Public defence
2016-10-06, KB3A9, KBC-huset, Umeå, 10:00 (English)
Opponent
Supervisors
Available from: 2016-09-15 Created: 2016-09-09 Last updated: 2017-03-17Bibliographically approved

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Kindgren, PeterNorén, LouiseBarajas Lopez, Juan de DiosShaikhali, JehadStrand, Åsa

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Kindgren, PeterNorén, LouiseBarajas Lopez, Juan de DiosShaikhali, JehadStrand, Åsa
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