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Multivariate Modeling of Proteins Related to Trapezius Myalgia, a Comparative Study of Female Cleaners with or without Pain
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Rehabilitation Medicine, Department of Medicine and Health Sciences (IMH), Faculty of Health Sciences, Linköping University and Pain and Rehabilitation Centre, County Council of Östergötland, SE 581 85 Linköping, Sweden. (Rehabilitation medicine)
Rehabilitation Medicine, Department of Medicine and Health Sciences (IMH), Faculty of Health Sciences, Linköping University. (Rehabilitation medicine)
Rehabilitation Medicine, Department of Medicine and Health Sciences (IMH), Faculty of Health Sciences, Linköping University. (Rehabilitation medicine)
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2013 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 8, no 9, e73285- p.Article in journal (Refereed) Published
Abstract [en]

The prevalence of chronic trapezius myalgia is high in women with high exposure to awkward working positions, repetitive movements and movements with high precision demands. The mechanisms behind chronic trapezius myalgia are not fully understood. The purpose of this study was to explore the differences in protein content between healthy and myalgic trapezius muscle using proteomics. Muscle biopsies from 12 female cleaners with work-related trapezius myalgia and 12 pain free female cleaners were obtained from the descending part of the trapezius. Proteins were separated with two-dimensional differential gel electrophoresis (2D-DIGE) and selected proteins were identified with mass spectrometry. In order to discriminate the two groups, quantified proteins were fitted to a multivariate analysis: partial least square discriminate analysis. The model separated 28 unique proteins which were related to glycolysis, the tricaboxylic acid cycle, to the contractile apparatus, the cytoskeleton and to acute response proteins. The results suggest altered metabolism, a higher abundance of proteins related to inflammation in myalgic cleaners compared to healthy, and a possible alteration of the contractile apparatus. This explorative proteomic screening of proteins related to chronic pain in the trapezius muscle provides new important aspects of the pathophysiology behind chronic trapezius myalgia.

Place, publisher, year, edition, pages
Public Library of Science , 2013. Vol. 8, no 9, e73285- p.
Keyword [en]
human, muscle, proteomics, pain, 2D-DIGE, trapezius, myalgia
National Category
Research subject
URN: urn:nbn:se:umu:diva-60613DOI: 10.1371/journal.pone.0073285ISI: 000324515600091OAI: diva2:561633
Swedish Research Council, K2011-69X-21874-01-6Forte, Swedish Research Council for Health, Working Life and Welfare, 2009-1761, 2010-0913
Available from: 2012-10-19 Created: 2012-10-19 Last updated: 2014-02-17Bibliographically approved
In thesis
1. Applying proteomics and metabolomics for studying human skeletal muscle with a focus on chronic trapezius myalgia
Open this publication in new window or tab >>Applying proteomics and metabolomics for studying human skeletal muscle with a focus on chronic trapezius myalgia
2012 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[en]
Tillämpning av proteomiska och metabolomiska metoder på human skelettmuskel med inriktning mot kronisk trapezius myalgi
Abstract [en]

Work related musculoskeletal disorders are the dominating causes of reported ill-health in industrialized countries. These chronic pain conditions are one of the most costly public health problems in Europe and North America. When work related musculoskeletal disorders are considered to be of muscular origin and the trapezius muscle is affected, the common appellation is trapezius myalgia. Since little is known about the genesis or how it is maintained, it is of great importance to better understand the pathophysiology of trapezius myalgia; doing so will better enable recommendations for prevention, treatment and rehabilitation. Several hypotheses have been presented based on biochemical alterations in the muscle, suggesting increased signaling of inflammatory substances and altered metabolism. Previous research has not been able to present the comprehensive picture of the muscle in pain. Thus there is a demand for more comprehensive research regarding the biochemical milleu of the chronic trapezius muscle.

Proteomic and metabolomic methods allow non-targeted simultaneous analyses of a large number of proteins and metabolites. The main emphasis in this thesis is on a proteomic method, two-dimensional differential gel electrophoresis (2D-DIGE). The method is validated to human skeletal muscle biopsy research with laboratory specific settings. In the baseline study, there were 14 metabolic, contractile, structural and regulatory proteins that differed significantly in abundance when trapezius and vastus lateralis muscles were compared. Using the validated 2D-DIGE method and the baseline study, a comparison between healthy and myalgic muscles was made. Biopsies from female cleaners with and without myalgia were compared to obtain results from women with the same type of work exposure. In the multivariate model, 28 identified unique proteins separated healthy and myalgic muscle and were grouped according to function: metabolic (n=10), contractile (n=9), regulatory (n=3), structural (n=4), and other (n=2). Finally, a second screening method, metabolomics, was introduced to analyze differences in metabolite content as a complement to and verification of the proteomic results. Gas chromatography-mass spectrometry (GC-MS) was performed on muscle interstitial fluid samples obtained with microdialysis, and differences in the abundance of extracellular metabolites were revealed.

 The 2D-DIGE method is a reliable method to analyze human skeletal muscle. The outcomes of the proteomic analyses were dependant on the statistical approach. Systematic differences in protein and metabolite content were detected using a multivariate approach. Univariate analyses were used to analyze individual proteins for their significance. The significant proteins in the baseline study were predominately related to muscle fiber type which correlated with the differences in fiber type content between trapezius and vastus lateralis. The proteomic and metabolomics studies where myalgic and healthy muscles were compared provide us with new clues and new aspects regarding the pathophysiology of the myalgic muscle.

Technically advanced methods employed in the thesis enabled an explorative screening of proteins of relevance for the pathophysiology of the myalgic muscle. The results of these analyses may contribute to the formulation of future hypothesis that need to be further evaluated.

Place, publisher, year, edition, pages
Umeå: Umeå Universitet, 2012. 60 p.
Umeå University medical dissertations, ISSN 0346-6612 ; 1533
Trapezius myalgia, proteomics, 2D-DIGE, metabolomics, microdialysate, biopsies
National Category
Other Basic Medicine
Research subject
Human Anatomy
urn:nbn:se:umu:diva-61399 (URN)978-91-7459-515-4 (ISBN)
Public defence
2012-12-06, BiA 201, Biologihuset, Umeå Universitet, Umeå, 09:00 (English)
Available from: 2012-11-15 Created: 2012-11-12 Last updated: 2012-11-15Bibliographically approved

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