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A collection of INDEL markers for map-based cloning in seven Arabidopsis accessions
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).
Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Institutionen för fysiologisk botanik. Umeå universitet, Teknisk-naturvetenskapliga fakulteten, Umeå Plant Science Centre (UPSC).ORCID-id: 0000-0001-6031-005X
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2012 (Engelska)Ingår i: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 63, nr 7, s. 2491-2501Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The availability of a comprehensive set of resources including an entire annotated reference genome, sequenced alternative accessions, and a multitude of marker systems makes Arabidopsis thaliana an ideal platform for genetic mapping. PCR markers based on INsertions/DELetions (INDELs) are currently the most frequently used polymorphisms. For the most commonly used mapping combination, ColumbiaxLandsberg erecta (Col-0xLer-0), the Cereon polymorphism database is a valuable resource for the generation of polymorphic markers. However, because the number of markers available in public databases for accessions other than Col-0 and Ler-0 is extremely low, mapping using other accessions is far from straightforward. This issue arose while cloning mutations in the Wassilewskija (Ws-4) background. In this work, approaches are described for marker generation in Ws-4 x Col-0. Complementary strategies were employed to generate 229 INDEL markers. Firstly, existing Col-0/Ler-0 Cereon predicted polymorphisms were mined for transferability to Ws-4. Secondly, Ws-0 ecotype Illumina sequence data were analyzed to identify INDELs that could be used for the development of PCR-based markers for Col-0 and Ws-4. Finally, shotgun sequencing allowed the identification of INDELs directly between Col-0 and Ws-4. The polymorphism of the 229 markers was assessed in seven widely used Arabidopsis accessions, and PCR markers that allow a clear distinction between the diverged Ws-0 and Ws-4 accessions are detailed. The utility of the markers was demonstrated by mapping more than 35 mutations in a Col-0xWs-4 combination, an example of which is presented here. The potential contribution of next generation sequencing technologies to more traditional map-based cloning is discussed.

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2012. Vol. 63, nr 7, s. 2491-2501
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Genetik Botanik
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URN: urn:nbn:se:umu:diva-60773DOI: 10.1093/jxb/err422ISI: 000304052300009OAI: oai:DiVA.org:umu-60773DiVA, id: diva2:562806
Tillgänglig från: 2012-10-26 Skapad: 2012-10-26 Senast uppdaterad: 2018-06-08Bibliografiskt granskad

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Pacurar, Daniel IoanStreet, NathanielBellini, Catherine

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Pacurar, Daniel IoanStreet, NathanielBellini, Catherine
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Institutionen för fysiologisk botanikUmeå Plant Science Centre (UPSC)
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Journal of Experimental Botany
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