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Nanoflow electrospinning serial femtosecond crystallography
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2012 (English)In: Acta Crystallographica Section D: Biological Crystallography, ISSN 0907-4449, E-ISSN 1399-0047, Vol. 68, no Pt 11, 1584-1587 p.Article in journal (Refereed) Published
Abstract [en]

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min(-1) and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2012. Vol. 68, no Pt 11, 1584-1587 p.
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Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-60816DOI: 10.1107/S0907444912038152PubMedID: 23090408OAI: oai:DiVA.org:umu-60816DiVA: diva2:563396
Available from: 2012-10-30 Created: 2012-10-30 Last updated: 2017-12-07Bibliographically approved

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