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Mitochondrial involvement in sensory neuronal cell death and survival
University of Manchester.
University of Manchester.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. University of Manchester.
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2012 (English)In: Experimental Brain Research, ISSN 0014-4819, E-ISSN 1432-1106, Vol. 221, no 4, 357-367 p.Article in journal (Refereed) Published
Abstract [en]

Peripheral nerve injuries (PNI) are continuing to be an ever-growing socio-economic burden affecting mainly the young working population and the current clinical treatments to PNI provide a poor clinical outcome involving significant loss of sensation. Thus, our understanding of the underlying factors responsible for the extensive loss of the sensory cutaneous subpopulation in the dorsal root ganglia (DRG) that occurs following injury needs to be improved. The current investigations focus in identifying visual cues of mitochondria-related apoptotic events in the various subpopulations of sensory cutaneous neurons. Sensory neuronal subpopulations were identified using FastBlue retrograde labelling following axotomy. Specialised fluorogenic probes, MitoTracker Red and MitoTracker Orange, were employed to visualise the dynamic changes of the mitochondrial population of neurons. The results reveal a fragmented mitochondrial network in sural neurons following apoptosis, whereas a fused elongated mitochondrial population is present in sensory proprioceptive muscle neurons following tibial axotomy. We also demonstrate the neuroprotective properties of NAC and ALCAR therapy in vitro. The dynamic mitochondrial network breaks down following oxidative exposure to hydrogen peroxide (H2O2), but reinitiates fusion after NAC and ALCAR therapy. In conclusion, this study provides both qualitative and quantitative evidence of the susceptibility of sensory cutaneous sub-population in apoptosis and of the neuroprotective effects of NAC and ALCAR treatment on H2O2-challenged neurons.

Place, publisher, year, edition, pages
2012. Vol. 221, no 4, 357-367 p.
Keyword [en]
Peripheral nerve injury (PNI), Dorsal root ganglion (DRG), N-acetyl cysteine (NAC), Acetyl-L-carnitine (ALCAR), Mitochondria, Mitotracker Red
National Category
Cell and Molecular Biology
URN: urn:nbn:se:umu:diva-59705DOI: 10.1007/s00221-012-3179-4ISI: 000307983100001OAI: diva2:563822
Available from: 2012-10-31 Created: 2012-09-25 Last updated: 2013-10-25Bibliographically approved

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