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Alterations in the β flap and β' dock domains of the RNA polymerase abolish NusA-mediated feedback regulation of the metY-nusA-infB operon
Umeå University, Faculty of Medicine, Department of Medical Biochemistry and Biophysics.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Virology.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
2011 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 193, no 16, 4113-4122 p.Article in journal (Refereed) Published
Abstract [en]

The RimM protein in Escherichia coli is important for the in vivo maturation of 30S ribosomal subunits and a ΔrimM mutant grows poorly due to assembly and translational defects. These deficiencies are suppressed partially by mutations that increase the synthesis of another assembly protein, RbfA, encoded by the metY-nusA-infB operon. Among these suppressors are mutations in nusA that impair the NusA-mediated negative-feedback regulation at internal intrinsic transcriptional terminators of the metY-nusA-infB operon. We describe here the isolation of two new mutations, one in rpoB and one in rpoC (encoding the β and β' subunits of the RNA polymerase, respectively), that increase the synthesis of RbfA by preventing NusA from stimulating termination at the internal intrinsic transcriptional terminators of the metY-nusA-infB operon. The rpoB2063 mutation changed the isoleucine in position 905 of the β flap-tip helix to a serine, while the rpoC2064 mutation duplicated positions 415 to 416 (valine-isoleucine) at the base of the β' dock domain. These findings support previously published in vitro results, which have suggested that the β flap-tip helix and β' dock domain at either side of the RNA exit tunnel mediate the binding to NusA during transcriptional pausing and termination.

Place, publisher, year, edition, pages
2011. Vol. 193, no 16, 4113-4122 p.
Keyword [en]
ribosome maturation protein; escherichia-coli operon; termination factor-rho; messenger-rna; polynucleotide phosphorylase; transcription elongation; nucleotide-sequence; level expression; gene; rimm
National Category
Natural Sciences Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-61270DOI: 10.1128/JB.00196-11PubMedID: 21685293OAI: oai:DiVA.org:umu-61270DiVA: diva2:565430
Available from: 2012-11-07 Created: 2012-11-07 Last updated: 2017-12-07Bibliographically approved

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Bylund, Göran ONord, StefanWikström, P Mikael
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