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The Rift Valley Fever virus protein NSm and putative cellular protein interactions
Umeå University, Faculty of Medicine, Department of Clinical Microbiology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Clinical Bacteriology.
Umeå University, Faculty of Medicine, Department of Clinical Microbiology, Infectious Diseases.
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2012 (English)In: Virology Journal, ISSN 1743-422X, Vol. 9, 139- p.Article in journal (Refereed) Published
Abstract [en]

Rift Valley Fever is an infectious viral disease and an emerging problem in many countries of Africa and on the Arabian Peninsula. The causative virus is predominantly transmitted by mosquitoes and high mortality and abortion rates characterize outbreaks in animals while symptoms ranging from mild to life-threatening encephalitis and hemorrhagic fever are noticed among infected humans. For a better prevention and treatment of the infection, an increased knowledge of the infectious process of the virus is required. The focus of this work was to identify protein-protein interactions between the non-structural protein (NSm), encoded by the M-segment of the virus, and host cell proteins. This study was initiated by screening approximately 26 million cDNA clones of a mouse embryonic cDNA library for interactions with the NSm protein using a yeast two-hybrid system. We have identified nine murine proteins that interact with NSm protein of Rift Valley Fever virus, and the putative protein-protein interactions were confirmed by growth selection procedures and beta-gal activity measurements. Our results suggest that the cleavage and polyadenylation specificity factor subunit 2 (Cpsf2), the peptidyl-prolyl cistrans isomerase (cyclophilin)-like 2 protein (Ppil2), and the synaptosome-associated protein of 25 kDa (SNAP-25) are the most promising targets for the NSm protein of the virus during an infection.

Place, publisher, year, edition, pages
2012. Vol. 9, 139- p.
Keyword [en]
Rift Valley fever, NSm, Yeast two-hybrid, SNAP-25, Ppil2, Cpsf2
National Category
Infectious Medicine
Identifiers
URN: urn:nbn:se:umu:diva-61224DOI: 10.1186/1743-422X-9-139ISI: 000308610100001OAI: oai:DiVA.org:umu-61224DiVA: diva2:565635
Available from: 2012-11-08 Created: 2012-11-07 Last updated: 2012-11-08Bibliographically approved

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Engdahl, CeciliaNäslund, JonasLindgren, LenaAhlm, ClasBucht, Göran
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