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Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). (Hultmark)
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2012 (English)In: PLoS pathogens, ISSN 1553-7374, Vol. 8, no 8, e1002872- p.Article in journal (Refereed) Published
Abstract [en]

RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense.

Place, publisher, year, edition, pages
2012. Vol. 8, no 8, e1002872- p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:umu:diva-61306DOI: 10.1371/journal.ppat.1002872PubMedID: 22916019OAI: diva2:565801
Available from: 2012-11-08 Created: 2012-11-08 Last updated: 2012-11-27Bibliographically approved

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Sadanandan, Sajna AEkström, Jens-OlaHultmark, Dan
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