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Gene expression of Mycobacterium tuberculosis putative transcription factors whiB1-7 in redox environments
Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
Center for Tuberculosis Research, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America.
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2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 7, e37516- p.Article in journal (Refereed) Published
Abstract [en]

The seven WhiB proteins of Mycobacterium tuberculosis (M.tb) are widely believed to be redox-sensing transcription factors due to their binding of iron-sulfur clusters and similarities to DNA binding proteins. Here, we explored the nature of this hypothesized relationship. We exposed M.tb to physiologic conditions such as gradual hypoxia, nitric oxide (NO), cyclic AMP and in vivo conditions, and measured transcription of the whiB genes. We found whiB3 to be induced both by hypoxia and NO, whiB7 to be induced in macrophage-like cells, and whiB4 to be induced in mouse lung. Cyclic AMP induced whiB1,-2, -4, -6 and -7. Our data indicate that the M.tb whiB genes are induced independently by various stimuli which may add versatility to their suggested redox-sensing properties.

Place, publisher, year, edition, pages
2012. Vol. 7, no 7, e37516- p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:umu:diva-61865DOI: 10.1371/journal.pone.0037516ISI: 000306956300004PubMedID: 22829866OAI: oai:DiVA.org:umu-61865DiVA: diva2:572419
Funder
Swedish Research Council
Note

This work was supported by The Swedish Research Council (VR) www.vr.se, the Swedish Society for Medical Research (SSMF) www.ssmf.se, and the National Institute of Health (NIH) www.nih.gov AI36973 and AI37856.

Available from: 2012-11-27 Created: 2012-11-27 Last updated: 2017-12-07Bibliographically approved

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