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Enhanced biofilm formation by Escherichia coli LPS mutants defective in hep biosynthesis
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). (Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, Japan)
Umeå University, Faculty of Science and Technology, Department of Chemistry.
Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR). Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS).
Umeå University, Faculty of Medicine, Molecular Infection Medicine Sweden (MIMS). Umeå University, Faculty of Medicine, Umeå Centre for Microbial Research (UCMR).
2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 12, e51241- p.Article in journal (Refereed) Published
Abstract [en]

Lipopolysaccharide (LPS) is the major component of the surface of Gram-negative bacteria and its polysaccharide portion is situated at the outermost region. We investigated the relationship between the polysaccharide portion of LPS and biofilm formation using a series of Escherichia coli mutants defective in genes earlier shown to affect the LPS sugar compositions. Biofilm formation by a deep rough LPS mutant, the hldE strain, was strongly enhanced in comparison with the parental strain and other LPS mutants. The hldE strain also showed a phenotype of increased auto-aggregation and stronger cell surface hydrophobicity compared to the wild-type. Similar results were obtained with another deep rough LPS mutant, the waaC strain whose LPS showed same molecular mass as that of the hldE strain. Confocal laser scanning microscopy (CLSM) analysis and biofilm formation assay using DNase I revealed that biofilm formation by the hldE strain was dependent on extracellular DNA. Furthermore, a loss of flagella and an increase in amount of outer membrane vesicles in case of the hldE strain were also observed by transmission electron microscopy and atomic force microscopy, respectively. In addition, we demonstrated that a mutation in the hldE locus, which alters the LPS structure, caused changes in both expression and properties of several surface bacterial factors involved in biofilm formation and virulence. We suggest that the implication of these results should be considered in the context of biofilm formation on abiotic surfaces, which is frequently associated with nosocominal infections such as the catheter-associated infections.

Place, publisher, year, edition, pages
Public library of science , 2012. Vol. 7, no 12, e51241- p.
Keyword [en]
pseudomonas-aeruginosa pao1; o-antigen lipopolysaccharide; negative antivirulence drugs; mouse large-intestine; extracellular dna; porphyromonas-gingivalis; salmonella-typhimurium; klebsiella-pneumoniae; monoclonal-antibody; membrane-vesicles
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-63741DOI: 10.1371/journal.pone.0051241ISI: 000313051500008PubMedID: 23284671OAI: oai:DiVA.org:umu-63741DiVA: diva2:582936
Available from: 2013-01-07 Created: 2013-01-07 Last updated: 2017-12-06Bibliographically approved

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Nakao, RyomaRamstedt, MadeleineWai, Sun NyuntUhlin, Bernt Eric

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Nakao, RyomaRamstedt, MadeleineWai, Sun NyuntUhlin, Bernt Eric
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Molecular Infection Medicine Sweden (MIMS)Umeå Centre for Microbial Research (UCMR)Department of Chemistry
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